Determination of glycemic monitoring marker 1,5-anhydroglucitol in plasma by liquid chromatography-electrospray tandem mass spectrometry
文献类型：期刊论文
| 作者 | Li, Shuijun2,3; Heng, Xiaoxiang1; Sheng, Hongguang4; Wang, Yiping2 ; Yu, Chen3
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2008-11-15 |
| 卷号 | 875期号:2页码:459-464 |
| 关键词 | 1,5-Anhydroglucitol LC-MS/MS Glycemic monitoring marker Human plasma |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2008.09.033 |
| 文献子类 | Article |
| 英文摘要 | Previous studies have shown that plasma 1,5-anhydroglucitol (1,5-AG) is markedly reduced among diabetic patients and therefore serves as a sensitive marker for short-term glycemic control. The current study describes the development of the liquid chromatography negative ion electrospray tandem mass spectrometry (LC-MS/MS) method to measure 1,5-AG in human plasma. The samples were pre-treated with protein precipitation and an isotope-labeled internal standard was used. Chromatographic separation was achieved on amide column (150 mm x 2.0 mm i.d., 5 mu m) followed by detection with multiple reaction monitoring mode. Linearity, accuracy, precision, recovery, matrix effect, and stability were evaluated during method validation over the range of 1-50 mu g/mL. The validated method has been clinically applied among 159 type 2 diabetic patients and 290 control subjects. A marked reduction in 1,5-AG levels among the diabetic patients and significant between-gender difference in nondiabetic subjects were observed. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | TYPE-1 DIABETES-MELLITUS ; FULLY ENZYMATIC METHOD ; SERUM 1,5-ANHYDROGLUCITOL ; CLINICAL MARKER ; GLUCOSE ; 1,5-ANHYDRO-D-GLUCITOL ; GLYCOMARK(TM) ; EXCURSIONS ; COMPLICATIONS ; FRUCTOSE |
| 资助项目 | Health Bureau of Xuhui District, Shanghai, China[SHXH20070108] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000261272900018 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279434]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Yu, Chen |
| 作者单位 | 1.Jiangsu Univ, Coll Med, Dept Clin Med, Zhenjiang, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China; 3.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200031, Peoples R China; 4.Shanghai Xuhui Cent Hosp, Dept Endocrinol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shuijun,Heng, Xiaoxiang,Sheng, Hongguang,et al. Determination of glycemic monitoring marker 1,5-anhydroglucitol in plasma by liquid chromatography-electrospray tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,875(2):459-464. |
| APA | Li, Shuijun,Heng, Xiaoxiang,Sheng, Hongguang,Wang, Yiping,&Yu, Chen.(2008).Determination of glycemic monitoring marker 1,5-anhydroglucitol in plasma by liquid chromatography-electrospray tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,875(2),459-464. |
| MLA | Li, Shuijun,et al."Determination of glycemic monitoring marker 1,5-anhydroglucitol in plasma by liquid chromatography-electrospray tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 875.2(2008):459-464. |
入库方式： OAI收割
来源：上海药物研究所
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