Identification of AOSC-binding proteins in neurons
文献类型：期刊论文
| 作者 | Liu Ming2; Nie Qin2; Xin Xianliang1; Geng Meiyu1,2
|
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2008-11 |
| 卷号 | 26期号:4页码:394-399 |
| 关键词 | AOSC Alzheimer's disease marine oligosaccharide binding protein(s) |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-008-0394-8 |
| 文献子类 | Article |
| 英文摘要 | Acidic oligosaccharide sugar chain (AOSC), a D-mannuronic acid oligosaccharide, derived from brown algae polysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer's Disease (AD) drug candidate. The identification of AOSC-binding protein(s) in neurons is very important for understanding its action mechanism. To determine the binding protein(s) of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 (AP-2) and amyloid precursor protein (APP). These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities. |
| WOS关键词 | OLIGOSACCHARIDE SUGAR CHAIN ; AMYLOID PRECURSOR PROTEIN ; ACIDIC OLIGOSACCHARIDE ; BETA-PROTEIN ; CLATHRIN ; APP |
| 资助项目 | National Basic Research Program of China (973 Program)[2003CB716400] ; Natural Science Foundation of China for Distinguished Young Scholars[30725046] ; Natural Science Foundation of China[30472029] ; Natural Science Foundation of China[30772612] ; Chinese High technology Research and Development Program of China(863 Program)[2006AA090501] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3465907 |
| WOS记录号 | WOS:000262669100010 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279447]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Geng Meiyu |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China 2.Ocean Univ China, Dept Mol Pharmacol, Sch Med & Pharm, Qingdao 266003, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu Ming,Nie Qin,Xin Xianliang,et al. Identification of AOSC-binding proteins in neurons[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2008,26(4):394-399. |
| APA | Liu Ming,Nie Qin,Xin Xianliang,&Geng Meiyu.(2008).Identification of AOSC-binding proteins in neurons.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,26(4),394-399. |
| MLA | Liu Ming,et al."Identification of AOSC-binding proteins in neurons".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 26.4(2008):394-399. |
入库方式： OAI收割
来源：上海药物研究所
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