Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice
文献类型：期刊论文
| 作者 | Gao, Fei2; Liu, Xing1,3; Shen, Ziying2; Jia, Xiaohui2; He, Han1,3; Gao, Jing1,3; Wu, Jianhong1,3; Jiang, Chunhong4; Zhou, Hu1,3 ; Wang, Yiping2
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| 刊名 | FRONTIERS IN PHYSIOLOGY
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| 出版日期 | 2018-08-17 |
| 卷号 | 9 |
| 关键词 | andrographolide sulfonate acute lung injury iTRAQ quantitative proteomics immunohistochemistry |
| ISSN号 | 1664-042X |
| DOI | 10.3389/fphys.2018.00939 |
| 文献子类 | Article |
| 英文摘要 | Andrographolide sulfonate (Andro-S), a sulfonation derivative of andrographolide, is known to be effective in treating inflammation-related diseases, while the underlying mechanisms and global protein alterations in response to Andro-S remain unknown. This study aimed to investigate the pharmacological effects and potential targets of Andro-S in a murine model of acute lung injury (ALI). ALI was induced by aerosolized lipopolysaccharide (LPS) exposure before treatment with Andro-S. Inflammatory state of each treatment group was determined by histological analysis and quantification of inflammatory markers. Differentially expressed proteins in lung tissues were identified by an iTRAQ-based quantitative proteomic approach and further confirmed by immunohistochemistry analysis. Administration of Andro-S alleviated LPS-induced histological changes in the lung and reduced the expression of inflammatory markers in serum, bronchoalveolar fluid and lung tissues. Proteomic analysis identified 31 differentially expressed proteins from a total of 2,234 quantified proteins in the lung. According to bioinformatics analysis, neutrophil elastase (ELANE), cathepsin G (CTSG) and myeloperoxidase (MPO), three neutrophil-derived proteases related to immune system process and defense responses to fungi were chosen as potential targets of Andro-S. Further immunohistochemistry analysis confirmed the inhibitory effects of Andro-S on LPS-induced ELANE, CTSG and MPO up-regulation. These results indicate that Andro-S suppressed the severity of LPS-induced ALI, possibly by attenuating the expression of and neutrophil-derived proteases. |
| WOS关键词 | SPECTROMETRY-BASED PROTEOMICS ; MOUTH-DISEASE HFMD ; MASS-SPECTROMETRY ; ORAL BIOAVAILABILITY ; CATHEPSIN-G ; IN-VITRO ; KAPPA-B ; PROTEIN ; QUANTIFICATION ; IDENTIFICATION |
| 资助项目 | National Natural Science Foundation of China[31500110] ; National Natural Science Foundation of China[21375138] ; National Natural Science Foundation of China[31570830] ; Open Project Program of State Key Laboratory of Innovative Natural Medicine and TCM Injections[QFSKL2017005] |
| WOS研究方向 | Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000441950700001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279616]  |
| 专题 | 分析化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药理学第一研究室 |
| 通讯作者 | Zhou, Hu; Wang, Yiping |
| 作者单位 | 1.Univ Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai, Peoples R China; 4.State Key Lab Innovat Nat Med & TCM Inject, Ganzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Fei,Liu, Xing,Shen, Ziying,et al. Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice[J]. FRONTIERS IN PHYSIOLOGY,2018,9. |
| APA | Gao, Fei.,Liu, Xing.,Shen, Ziying.,Jia, Xiaohui.,He, Han.,...&Wang, Yiping.(2018).Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice.FRONTIERS IN PHYSIOLOGY,9. |
| MLA | Gao, Fei,et al."Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice".FRONTIERS IN PHYSIOLOGY 9(2018). |
入库方式： OAI收割
来源：上海药物研究所
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