Development and validation of a highly sensitive LC-MS/MS method for determination of brain active agent dianhydrogalactitol in mouse plasma and tissues: Application to a pharmacokinetic study
文献类型：期刊论文
| 作者 | Sun, Lihan1,2; Wu, Huajing1,6; Ren, Lianjie3; Cong, Jiaojiao1,2; Qin, Xiaohui1,2; Wang, Xingli4; Qi, Xinming6 ; Kang, Lifeng5; Zhang, Junying4; Wu, Chunyong1,2
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2018-06-15 |
| 卷号 | 1087页码:90-97 |
| 关键词 | 1,2:5,6-dianhydrogalactitol Derivatization LC-MS/MS Plasma Tissue distribution |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2018.04.026 |
| 文献子类 | Article |
| 英文摘要 | A sensitive and specific liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantitative analysis of 1,2:5,6-dianhydrogalactitol (DAG) in mouse plasma and tissues. Sodium diethyldithiocarbamate (DDTC) was used as the derivatization reagent to improve its LC-MS/MS behavior. Analytes were separated on a Welch Ultimate XB-CN column with a mobile phase consisting of acetonitrile and 0.1% formic acid solution (65:35). The MS analysis was conducted by positive electrospray ionization in multiple-reaction monitoring (MRM) mode. Good linearity (r(2) > 0.9958) was observed over the concentration range of 1-1000 ng/mL in plasma and tissue homogenates (brain, liver, heart, spleen, lung and kidney). The intra- and inter-batch precision and accuracy of DAG in plasma and brain samples were all within the acceptable limits. The extraction recovery was stable and no significant matrix effects were observed. The method was successfully applied to study the pharmacokinetic and tissue distribution of DAG in mice after intravenous administration. DAG could cross the blood-brain barrier and had limited liver distribution. Rat primary hepatocytes in vitro experiments demonstrated that DAG had a safe profile in liver. |
| WOS关键词 | CENTRAL-NERVOUS-SYSTEM ; CHROMATOGRAPHIC ANALYSIS ; LIVER-CELLS ; IN-VITRO ; RAT ; GLIOBLASTOMA ; HEPATOCYTES ; DISPOSITION ; NSC-132313 ; INJURY |
| 资助项目 | National Natural Science Foundation of China[81673681] ; National Natural Science Foundation of China[81473357] ; Natural Science Foundation of Jiangsu Province[BK2012358] ; Specialized Research Fund for the Doctoral Program of Higher Education[20120096120004] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000432761400012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279704]  |
| 专题 | 药物安全性评价中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhang, Junying; Wu, Chunyong |
| 作者单位 | 1.China Pharmaceut Univ, Dept Pharmaceut Anal, Nanjing 210009, Jiangsu, Peoples R China; 2.China Pharmaceut Univ, Key Lab Drug Qual Control & Pharmacovigilance, Minist Educ, Nanjing 210009, Jiangsu, Peoples R China; 3.China Food & Drug Adm, Ctr Drug Evaluat, Beijing 100038, Peoples R China; 4.China Pharmaceut Univ, Dept TCMs Pharmaceut, Nanjing 210009, Jiangsu, Peoples R China; 5.Univ Sydney, Fac Pharm, Sydney, NSW 2006, Australia 6.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, State Key Lab Drug Res, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Sun, Lihan,Wu, Huajing,Ren, Lianjie,et al. Development and validation of a highly sensitive LC-MS/MS method for determination of brain active agent dianhydrogalactitol in mouse plasma and tissues: Application to a pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2018,1087:90-97. |
| APA | Sun, Lihan.,Wu, Huajing.,Ren, Lianjie.,Cong, Jiaojiao.,Qin, Xiaohui.,...&Wu, Chunyong.(2018).Development and validation of a highly sensitive LC-MS/MS method for determination of brain active agent dianhydrogalactitol in mouse plasma and tissues: Application to a pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1087,90-97. |
| MLA | Sun, Lihan,et al."Development and validation of a highly sensitive LC-MS/MS method for determination of brain active agent dianhydrogalactitol in mouse plasma and tissues: Application to a pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1087(2018):90-97. |
入库方式： OAI收割
来源：上海药物研究所
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