Constituents of Mediterranean Spices Counteracting Vascular Smooth Muscle Cell Proliferation: Identification and Characterization of Rosmarinic Acid Methyl Ester as a Novel Inhibitor
文献类型：期刊论文
| 作者 | Liu, Rongxia2; Heiss, Elke H.3; Waltenberger, Birgit4,5; Blazevic, Tina3; Schachner, Daniel3; Jiang, Baohong1 ; Krystof, Vladimir9,10; Liu, Wanhui2; Schwaiger, Stefan4,5; Pena-Rodriguez, Luis M.8
|
| 刊名 | MOLECULAR NUTRITION & FOOD RESEARCH
 |
| 出版日期 | 2018-04 |
| 卷号 | 62期号:7 |
| 关键词 | Mediterranean spices neointima formation rosmarinic acid rosmarinic acid methyl ester vascular smooth muscle cells |
| ISSN号 | 1613-4125 |
| DOI | 10.1002/mnfr.201700860 |
| 文献子类 | Article |
| 英文摘要 | Scope: Aberrant vascular smooth muscle cell (VSMC) proliferation is involved in atherosclerotic plaque formation and restenosis. Mediterranean spices have been reported to confer cardioprotection, but their direct influence on VSMCs has largely not been investigated. This study aims at examining rosmarinic acid (RA) and 11 related constituents for inhibition of VSMC proliferation in vitro, and at characterizing the most promising compound for their mode of action and influence on neointima formation in vivo. Methods and results: RA, rosmarinic acid methyl ester (RAME), and caffeic acid methyl ester inhibit VSMC proliferation in a resazurin conversion assay with IC(50)s of 5.79, 3.12, and 6.78 mu m, respectively. RAME significantly reduced neointima formation in vivo in a mouse femoral artery cuff model. Accordingly, RAME leads to an accumulation of VSMCs in the G(0)/G(1) cell-cycle phase, as indicated by blunted retinoblastoma protein phosphorylation upon mitogen stimulation and inhibition of cyclin-dependent kinase 2 in vitro. Conclusion: RAME represses PDGF-induced VSMC proliferation in vitro and reduces neointima formation in vivo. These results recommend RAME as an interesting compound with VSMC-inhibiting potential. Future metabolism and pharmacokinetics studies might help to further evaluate the potential relevance of RAME and other spice-derived polyphenolics for vasoprotection. |
| WOS关键词 | FORMATION IN-VIVO ; NEOINTIMA FORMATION ; METABOLITES ; INDIRUBIN-3'-MONOXIME ; ATHEROSCLEROSIS ; PLUMERICIN ; ACTIVATOR ; CASS. ; VITRO |
| 资助项目 | Herzfelder Familienstiftung[00000000] ; National Natural Science Foundation of China[81603326] ; Austrian Science Fund (FWF)[P25971-B23] ; European Union[252881] ; European Union[909881] |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000429090900005 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279832]  |
| 专题 | 上海中药现代化研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Liu, Rongxia; Atanasov, Atanas G. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 2.Univ Yantai, Sch Pharm, Key Lab Mol Pharmacol & Drug Evaluat, Minist Educ,Collaborat Innovat Ctr Adv Drug Deliv, Yantai, Peoples R China; 3.Univ Vienna, Dept Pharmacognosy, Vienna, Austria; 4.Univ Innsbruck, Inst Pharm Pharmacognosy, Innsbruck, Austria; 5.Univ Innsbruck, CMBI, Innsbruck, Austria; 6.Polish Acad Sci, Inst Genet & Anim Breeding, Jastrzebiec, Poland 7.Med Univ Vienna, Inst Vasc Biol & Thrombosis Res, Ctr Physiol & Pharmacol, Vienna, Austria; 8.Ctr Invest Cient Yucatan, Unidad Biotecnol, Merida, Mexico; 9.Inst Expt Bot AS CR, Olomouc, Czech Republic; 10.Palacky Univ, Lab Growth Regulators, Olomouc, Czech Republic; |
| 推荐引用方式 GB/T 7714 | Liu, Rongxia,Heiss, Elke H.,Waltenberger, Birgit,et al. Constituents of Mediterranean Spices Counteracting Vascular Smooth Muscle Cell Proliferation: Identification and Characterization of Rosmarinic Acid Methyl Ester as a Novel Inhibitor[J]. MOLECULAR NUTRITION & FOOD RESEARCH,2018,62(7). |
| APA | Liu, Rongxia.,Heiss, Elke H..,Waltenberger, Birgit.,Blazevic, Tina.,Schachner, Daniel.,...&Atanasov, Atanas G..(2018).Constituents of Mediterranean Spices Counteracting Vascular Smooth Muscle Cell Proliferation: Identification and Characterization of Rosmarinic Acid Methyl Ester as a Novel Inhibitor.MOLECULAR NUTRITION & FOOD RESEARCH,62(7). |
| MLA | Liu, Rongxia,et al."Constituents of Mediterranean Spices Counteracting Vascular Smooth Muscle Cell Proliferation: Identification and Characterization of Rosmarinic Acid Methyl Ester as a Novel Inhibitor".MOLECULAR NUTRITION & FOOD RESEARCH 62.7(2018). |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。