中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 化学蛋白质组学研究中心
USP9X controls translation efficiency via deubiquitination of eukaryotic translation initiation factor 4A1

文献类型：期刊论文

作者Li, Zengxia3,4,5; Cheng, Zhao3,4,5; Raghothama, Chaerkady7,8; Cui, Zhaomeng3,4,5; Liu, Kaiyu3,4,5; Li, Xiaojing3,4,5; Jiang, Chenxiao3,4,5; Jiang, Wei3,4,5; Tan, Minjia9; Ni, Xiaohua1
刊名NUCLEIC ACIDS RESEARCH
出版日期2018-01-25
卷号46期号:2页码:823-839
ISSN号0305-1048
DOI10.1093/nar/gkx1226
文献子类Article
英文摘要Controlling translation initiation is an efficient way to regulate gene expression at the post-transcriptional level. However, current knowledge regarding regulatory proteins and their modes of controlling translation initiation is still limited. In this study, we employed tandem affinity purification and mass spectrometry to screen for unknown proteins associated with the translation initiation machinery. Ubiquitin specific peptidase 9, X-linked (USP9X), was identified as a novel binding partner, that interacts with the eukaryotic translation initiation factor 4B (eIF4B) in a mRNA-independent manner. USP9X-deficient cells presented significantly impaired nascent protein synthesis, cap-dependent translation initiation and cellular proliferation. USP9X can selectively alter the translation of pro-oncogenic mRNAs, such as cMyc and XIAP. Moreover, we found that eIF4A1, which is primarily ubiquitinated at Lys-369, is the substrate of USP9X. USP9X dysfunction increases the ubiquitination of eIF4A1 and enhances its degradation. Our results provide evidence that USP9X is a novel regulator of the translation initiation process via deubiquitination of eIF4A1, which offers new insight in understanding the pivotal role of USP9X in human malignancies and neurodevelopmental disorders.
WOS关键词FACTOR 4B ; PROSTATE-CANCER ; CELL-SURVIVAL ; PHOSPHORYLATION ; EIF4B ; UBIQUITINATION ; PROTEIN ; FAM ; DEGRADATION ; PROTEOMICS
资助项目National Natural Science Foundation of China[31270830] ; National Natural Science Foundation of China[21572038] ; National Natural Science Foundation of China[81372768] ; Fund of the State Key Laboratory of Bioorganic and Natural Products Chemistry[00000000] ; Fund of the State Key Laboratory of Drug Research, Chinese Academy of Science[SIMM1601KF-08]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000423812300031
出版者OXFORD UNIV PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/279945]  
专题化学蛋白质组学研究中心
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Dang, Yongjun
作者单位1.Fudan Univ, Key Lab Reprod Regulat NPFPC, SIPPR, IAD, Shanghai 200032, Peoples R China;
2.Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA;
3.Fudan Univ, Key Lab Mol Med, Minist Educ, Shanghai 200032, Peoples R China;
4.Fudan Univ, Dept Biochem & Mol Biol, Shanghai Med Coll, Shanghai 200032, Peoples R China;
5.Fudan Univ, Dept Pulm & Crit Care Med, Huashan Hosp, Shanghai 200032, Peoples R China;
6.Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21205 USA
7.Johns Hopkins Univ, McKusick Nathans Inst Genet Med, Baltimore, MD 21205 USA;
8.Johns Hopkins Univ, Dept Biol Chem, Baltimore, MD 21205 USA;
9.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Li, Zengxia,Cheng, Zhao,Raghothama, Chaerkady,et al. USP9X controls translation efficiency via deubiquitination of eukaryotic translation initiation factor 4A1[J]. NUCLEIC ACIDS RESEARCH,2018,46(2):823-839.
APA Li, Zengxia.,Cheng, Zhao.,Raghothama, Chaerkady.,Cui, Zhaomeng.,Liu, Kaiyu.,...&Dang, Yongjun.(2018).USP9X controls translation efficiency via deubiquitination of eukaryotic translation initiation factor 4A1.NUCLEIC ACIDS RESEARCH,46(2),823-839.
MLA Li, Zengxia,et al."USP9X controls translation efficiency via deubiquitination of eukaryotic translation initiation factor 4A1".NUCLEIC ACIDS RESEARCH 46.2(2018):823-839.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。