Simultaneous determination of huperzine A and huperzine B in different parts of Huperzia serrata from different habitats by HPLC
文献类型：期刊论文
| 作者 | Yuan Jingquan2; Zhou Xiaolei2; Wang Shuo2; Yan Zhigang2; Feng Shixin2; Jiang Jiaomei2; Zhu Dayuan1; Ma Xiaojun2 |
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2012 |
| 卷号 | 32期号:9页码:1541 |
| 关键词 | Huperzia serrata Qiancengta huperzine A huperzine B HPLC composition analysis of root,leaf and stem traditional Chinese medicine quality control different habitats quality evaluation |
| ISSN号 | 0254-1793 |
| 其他题名 | HPLC测定不同产地与不同部位千层塔中石杉碱甲和石杉碱乙含量 |
| 文献子类 | Article |
| 英文摘要 | Objective:To develop an HPLC method for simultaneous determination of huperzine A(Hup-A) and huperzine B(Hup-B) in different parts of Huperzia serrate from different habitats.Methods:The assay was performed on an UItimate XB-C_(18)(4.6 mm*250 mm,5 mum) column with the mobile phase of acetonitrile-methanol-water(10∶ 55∶ 240) containing 0.32 % phosphoric acid and 0.5 % triethylamine.The flow rate was 1.0 mL·min~(-1),the detection wavelength was set at 309 nm,and the column temperature was set at 25 ℃.Results:The contents of huperzine A and huperzine B in Huperzia serrata collected at different habitats were evidently different.The maximum contents of huperzine A and huperzine B were 0.0900% and 0.0183%,respectively;the minimum contents of huperzine A and huperzine B were 0.0014% and 0,respectively.Conclusions:The method is simple,accurate and repeatable,which provides a reliable way for evaluating the quality of Huperzia serrata by measuring huperzine A and huperzine B,as well as provide a reference for the determination of the best origin and the most rational part of the plant. |
| 资助项目 | 广西应用基础研究专项[00000000] ; 广西科技攻关项目[00000000] ; 广西青年基金项目[00000000] ; 广西医疗卫生重点课题[00000000] ; 南宁市科技攻关项目[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4628814 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267820]  |
| 专题 | 天然药物化学研究室 |
| 作者单位 | 1.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. 2.Guangxi Institute of Medicinal Plant, Nanning, Guangxi 530023, China.; |
| 推荐引用方式 GB/T 7714 | Yuan Jingquan,Zhou Xiaolei,Wang Shuo,et al. Simultaneous determination of huperzine A and huperzine B in different parts of Huperzia serrata from different habitats by HPLC[J]. Chinese Journal of Pharmaceutical Analysis,2012,32(9):1541. |
| APA | Yuan Jingquan.,Zhou Xiaolei.,Wang Shuo.,Yan Zhigang.,Feng Shixin.,...&Ma Xiaojun.(2012).Simultaneous determination of huperzine A and huperzine B in different parts of Huperzia serrata from different habitats by HPLC.Chinese Journal of Pharmaceutical Analysis,32(9),1541. |
| MLA | Yuan Jingquan,et al."Simultaneous determination of huperzine A and huperzine B in different parts of Huperzia serrata from different habitats by HPLC".Chinese Journal of Pharmaceutical Analysis 32.9(2012):1541. |
入库方式： OAI收割
来源：上海药物研究所
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