Effects of GHSC-73 on proliferation and cell cycle of human hepatocellular carcinoma HepG2 cells
文献类型：期刊论文
| 作者 | FENG Bo1; GUO Yue-wei2 ; HUANG Cai-guo1; LI Liang2; JIAO Bing-hua1
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| 刊名 | Academic Journal of Second Military Medical University
 |
| 出版日期 | 2009 |
| 卷号 | 30期号:10页码:1110-1114 |
| 关键词 | GHSC-73 liver neoplasms cardiac glycosidess GHSC-73 cell proliferation cell cycle |
| ISSN号 | 0258-879X |
| 其他题名 | GHSC-73对人肝癌细胞株HepG2细胞增殖及细胞周期的影响 |
| 文献子类 | Article |
| 英文摘要 | Objective:To investigate the effects of beta-D-glucosyl-(1-4)-alpha-L-thevetosides of 17beta-digitoxigenin (GHSC-73) ,an isolate from the seeds of Cerbera manghas L. ,on cell growth and cell cycle regulation of human hepatocellular carcinoma cell line HepG2,and to discuss the related mechanism. Methods: HepG2 cells were treated with different concentrations (0-80 mumol/L) of GHSC-73. Cell viability was determined using MTT assay at 24,48, and 72 h after treatment. Cell cycle distribution was assessed by flow cytometry after propidium iodide (PD staining. Expression of the S phase associated genes GADD153,cyclin D1,cyclin A2, DHFR, TYMS, and p21 was determined by real-time RT-PCR before and after GHSC-73 treatment. Results: GHSC-73 inhibited the cell proliferation of HepG2 cells in a dose- and time-dependent manner. The values of IC_(50) were (5. 18 0. 21) ,(0. 37 + 0. 08) ,and (1. 660. 16) mumol/L at 24,48,and 72 h, respectively. Compared with control group,the proportion of cells in S phase increased in the treatment group with the prolongation of treatment, the cells in G_0/G_1 phase gradually decreased (P<0. 05),and cells in G_2/M phase remained unchanged,indicating that GHSC-73 blocked HepG2 cell in S phase. Real-time RT-PCR showed that cyclin A2, DHFR, and TYMS were down-regulated,and p21,GADD153, and cyclin D1 were up-regulated, which might be associated with the GHSC-73-induced S phase arrest in HepG2 cells. Conclusion: GHSC-73 can inhibit growth of HepG2 cells by inducing S phase arrests down-regulation of cyclin A2,DHFR, and TYMS genes and up-regulation of p21 ,GADD153,and cyclin Dl genes might participate in the induction of arrest. |
| 资助项目 | 国家高技术研究发展计划("863"计划)[00000000] |
| WOS研究方向 | General & Internal Medicine ; Oncology (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3682236 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268020]  |
| 专题 | 天然药物化学研究室 上海药物代谢研究中心 |
| 作者单位 | 1.Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China.; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | FENG Bo,GUO Yue-wei,HUANG Cai-guo,et al. Effects of GHSC-73 on proliferation and cell cycle of human hepatocellular carcinoma HepG2 cells[J]. Academic Journal of Second Military Medical University,2009,30(10):1110-1114. |
| APA | FENG Bo,GUO Yue-wei,HUANG Cai-guo,LI Liang,&JIAO Bing-hua.(2009).Effects of GHSC-73 on proliferation and cell cycle of human hepatocellular carcinoma HepG2 cells.Academic Journal of Second Military Medical University,30(10),1110-1114. |
| MLA | FENG Bo,et al."Effects of GHSC-73 on proliferation and cell cycle of human hepatocellular carcinoma HepG2 cells".Academic Journal of Second Military Medical University 30.10(2009):1110-1114. |
入库方式： OAI收割
来源：上海药物研究所
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