Inhibition of xathine oxidase by rosmarinic acid
文献类型：期刊论文
| 作者 | SHANG Yanjun1; HUANG Caiguo1; JIANG Sanhao2; ZHU Dayuan2; WEI Shanjian1; JIAO Binhua1 |
| 刊名 | Academic Journal of Second Military Medical University
 |
| 出版日期 | 2006 |
| 卷号 | 27期号:2页码:189-191 |
| 关键词 | rosmarinic acid xanthine oxidase uric acid superoxide anions apoptosis |
| ISSN号 | 0258-879X |
| 其他题名 | 迷迭香酸对黄嘌呤氧化酶的抑制作用 |
| 文献子类 | Article |
| 英文摘要 | Objective： To study the inhibitory effect of rosmarinic acid on xanthine oxidase. Methods： Xanthine oxidase （0.1 U/ml） was incubated with xanthine （1 mmol/L for determining formation of uric acid; 50mumol/L for determining superoxide anions） in the presence of 20, 40 and 60mug/ml rosmarinic acid or allopurinol as positive control. The formation of uric acid was determined by automatic biochemical analyzer 5 rain after reaction and the production of superoxide anions was measured by Nitro Blue Btetrazolium （NBT） reduction. HL-60 cells （1 ml, 2*10^5/ml） were pretreated with xanthine （100mul, 6 mol/L） and xanthine oxidase （100mul, 0.1 U/ml）, then rosmarinic acid （500mug/ml） or allopurinol （1mug/ml, as positive control） （AnnexinV-PI kit） was added to determine the cell apoptosis rate. HL-60 cells （1 ml, 2 * 10^5/ml） were also pretreated with xanthine （100 mul, 6 mol/L） and xanthine oxidase （100mul, 0.1 U/ml）, then rosmarinic acid （500 mug/ml） or SOD （100 U/ ml, as positive control） （cell cycle method） was added to determine the cell apoptosis rate. Results： Rosmarinic acid obviously inhibited the production of uric acid and superoxide anion-induced reaction in NBT assay, with their IC50being 56mug/ml and 21mug/ml, respectively. The rates of apoptosis inhibition by rosmarinic acid were both over 40% by Annexin V-PI kit and cell cycle method. Conclusion： Rosmarinic acid is a competitive inhibitor of xanthine oxidase. |
| 资助项目 | 国家自然科学基金[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2332265 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268267]  |
| 专题 | 天然药物化学研究室 |
| 作者单位 | 1.Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China.; 2.Shanghai Institute of Materia Medica, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | SHANG Yanjun,HUANG Caiguo,JIANG Sanhao,et al. Inhibition of xathine oxidase by rosmarinic acid[J]. Academic Journal of Second Military Medical University,2006,27(2):189-191. |
| APA | SHANG Yanjun,HUANG Caiguo,JIANG Sanhao,ZHU Dayuan,WEI Shanjian,&JIAO Binhua.(2006).Inhibition of xathine oxidase by rosmarinic acid.Academic Journal of Second Military Medical University,27(2),189-191. |
| MLA | SHANG Yanjun,et al."Inhibition of xathine oxidase by rosmarinic acid".Academic Journal of Second Military Medical University 27.2(2006):189-191. |
入库方式： OAI收割
来源：上海药物研究所
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