Investigating noncovalent interactions of rutin - serum albumin by capillary electrophoresis - frontal analysis
文献类型：期刊论文
| 作者 | Lu, Qin-hua; Ba, Chun-dan; Chen, Dong-ying
|
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2008-08-05 |
| 卷号 | 47期号:4-5页码:888-891 |
| 关键词 | capillary electrophoresis frontal analysis rutin serum albumin binding |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2008.03.007 |
| 文献子类 | Article |
| 英文摘要 | The application of capillary electrophoresis-frontal analysis (CE-FA) to study noncovalent interaction between rutin and serum albumin (bovine serum albumin, BSA and human serum albumin, HSA) in phosphate buffer solution (67 mM, pH 7.4) at 37 degrees C is presented. Using fixed HSA or BSA concentration and increasing rutin concentration, the number of primary binding sites per HSA or BSA molecules, and the affinity constants were obtained. Both affinity constants are in a comparable range suggesting the similarity of affinity properties of HSA and BSA towards rutin. The proposed CE-FA method is simple, rapid and cost-effective which may be useful in further high-throughput protein binding studies of multi-components in traditional herbal medicines for pharmacological effect evaluations. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | BINDING CONSTANTS ; FLAVONOIDS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000257220300036 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272844]  |
| 专题 | 药物质量控制与固体化学研究中心 |
| 通讯作者 | Chen, Dong-ying |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Qin-hua,Ba, Chun-dan,Chen, Dong-ying. Investigating noncovalent interactions of rutin - serum albumin by capillary electrophoresis - frontal analysis[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2008,47(4-5):888-891. |
| APA | Lu, Qin-hua,Ba, Chun-dan,&Chen, Dong-ying.(2008).Investigating noncovalent interactions of rutin - serum albumin by capillary electrophoresis - frontal analysis.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,47(4-5),888-891. |
| MLA | Lu, Qin-hua,et al."Investigating noncovalent interactions of rutin - serum albumin by capillary electrophoresis - frontal analysis".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 47.4-5(2008):888-891. |
入库方式： OAI收割
来源：上海药物研究所
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