Development and validation of a sensitive liquid chromatography-tandem mass spectrometry method for the determination of paeoniflorin in rat brain and its application to pharmacokinetic study
文献类型：期刊论文
| 作者 | Xia, Su-Mei; Shen, Rong; Sun, Xue-Ying; Shen, Li-Li; Yang, Yi-Ming; Ke, Yin; Wang, Yun; Chen, Dong-Ying ; Han, Xing-Mei
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2007-09-15 |
| 卷号 | 857期号:1页码:32-39 |
| 关键词 | LC-MS/MS paeoniflorin brain distribution matrix effects pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2007.06.022 |
| 文献子类 | Article |
| 英文摘要 | A sensitive and specific method was developed and validated for the determination of paeoniflorin in rat brain with liquid chromatography-tandem mass spectrometry. Sample pretreatment involved protein precipitation following solid-phase extraction. Paeoniflorin and geniposide (internal standard) were separated isocratically on a Waters Symmetry C 18 column (150 mm x 2.1 min i.d., 5 mu m), using a mobile phase of rnethanol/water with 0.1% formic acid (50:50. v/v) at a flow-rate of 200-300 mu L/min in 4 min. A Finngan LTQ tandem mass spectrometer equipped with electrospray ionization source was operated in the positive ion mode. Selective reaction monitoring was performed to quantify paeoniflorin and the internal standard at m/z transitions of 503 -> 381 and 411 -> 23 1. respectively. A good linearity was found in the range of 2-500 ng/mL (R-2 = 0.9939). The intra- and inter-batch assay precisions (coefficient of variation. CV) at 5.50 and 400 ng/mL (n = 5) ranged from 6.3% to 9.7% and 1.2% to 7.2%. respectively. and the accuracies were from 95.9% to 101.6% and 99.4% to 102.9%, respectively. The mean recoveries of paeoniflorin were 81.2%. 80.9% and 82.3% at 5.50 and 400 ng/mL (n = 5). respectively, and the mean recovery of the internal standard was 76.7% with a concentration of 50 ng/mL (n = 5). Stability studies showed that paeoniflorin was stable in different conditions. Finally. the method was successfully applied to the pharmacokinetic study of paeoniflorin in rat brain following a single subcutaneous administration ( 10 mg/kg) to rats. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | RADIAL MAZE PERFORMANCE ; SCOPOLAMINE-INDUCED DEFICIT ; ADENOSINE A(1) RECEPTOR ; SOLID-PHASE EXTRACTION ; PAEONIA-LACTIFLORA ; MAJOR CONSTITUENT ; DIAPHRAGM MUSCLE ; CRUDE DRUGS ; GLYCYRRHIZIN ; ROOT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000249874400005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273148]  |
| 专题 | 药物质量控制与固体化学研究中心 |
| 通讯作者 | Chen, Dong-Ying |
| 作者单位 | 1.Shanghai Pharmaceut Grp Ltd, Cent Res Inst, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Su-Mei,Shen, Rong,Sun, Xue-Ying,et al. Development and validation of a sensitive liquid chromatography-tandem mass spectrometry method for the determination of paeoniflorin in rat brain and its application to pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,857(1):32-39. |
| APA | Xia, Su-Mei.,Shen, Rong.,Sun, Xue-Ying.,Shen, Li-Li.,Yang, Yi-Ming.,...&Han, Xing-Mei.(2007).Development and validation of a sensitive liquid chromatography-tandem mass spectrometry method for the determination of paeoniflorin in rat brain and its application to pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,857(1),32-39. |
| MLA | Xia, Su-Mei,et al."Development and validation of a sensitive liquid chromatography-tandem mass spectrometry method for the determination of paeoniflorin in rat brain and its application to pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 857.1(2007):32-39. |
入库方式： OAI收割
来源：上海药物研究所
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