Assignment of proton resonances and conformational characterization of oligodeoxyribonucleic acid d(CCGTACGG) in solution
文献类型：期刊论文
| 作者 | Wang, P; Shi, GB; Song, GQ; Chen, KX ; Ji, RY
|
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 1996-11 |
| 卷号 | 28期号:6页码:703-705 |
| 关键词 | oligodeoxyribonucleic acid 2D NMR solution conformation |
| ISSN号 | 0582-9879 |
| 文献子类 | Article |
| 英文摘要 | Lycobetaine prepared from lycorine is a new anticancer agent. The experimental and quantum pharmacological studies revealed that lycobetaine can interact with DNA by intercalation, preferentially into GC base pairs. In order to provide detailed interaction model of lycobetaine-DNA, a self-complementary octanucleotide d(CCGTACGG) was designed and synthesized by using new HELP (high efficiency liquid phase) according to its nature, the sample was prepared to the desired final concentration by adding salt and buffer solutions. Two-dimensional H-1-H-1 COSY and NOESY spectra in 99.8% D2O and 95% H2O were recorded for the duplex, and the NMR techniques of presaturation and WATERGATE were applied to water suppression. Protons of every spin system were identified by their scalar couplings, then through their spacial couplings all protons in the molecule were assigned except the poorly resolved H5' and H5'' resonances. The chemical shifts of exchangeable protons and NOE intensities of nonexchangeable protons indicate qualitatively that the d(CCGTACGG) helix is right-handed B-DNA in aqueous solution. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 中文 |
| CSCD记录号 | CSCD:314217 |
| WOS记录号 | WOS:A1996WA13800020 |
| 出版者 | SHANGHAI INST BIOCHEMISTRY, ACADEMIA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274946]  |
| 专题 | 药物发现与设计中心 |
| 作者单位 | ACAD SINICA,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Wang, P,Shi, GB,Song, GQ,et al. Assignment of proton resonances and conformational characterization of oligodeoxyribonucleic acid d(CCGTACGG) in solution[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,1996,28(6):703-705. |
| APA | Wang, P,Shi, GB,Song, GQ,Chen, KX,&Ji, RY.(1996).Assignment of proton resonances and conformational characterization of oligodeoxyribonucleic acid d(CCGTACGG) in solution.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,28(6),703-705. |
| MLA | Wang, P,et al."Assignment of proton resonances and conformational characterization of oligodeoxyribonucleic acid d(CCGTACGG) in solution".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 28.6(1996):703-705. |
入库方式： OAI收割
来源：上海药物研究所
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