Application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction of captopril with human serum albumin in the absence and presence of hydrochlorothiazide
文献类型：期刊论文
| 作者 | Liu, Ting-Ting; Xiang, Li-Li; Wang, Jian-Ling; Chen, Dong-Ying
|
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2015-11-10 |
| 卷号 | 115页码:31-35 |
| 关键词 | Capillary electrophoresis-frontal analysis Binding interaction Captopril Hydrochlorothiazide Human serum albumin |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2015.06.022 |
| 文献子类 | Article |
| 英文摘要 | The application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction between antihypertensive drug captopril and human serum albumin in the absence and presence of diuretic drug hydrochlorothiazide was presented in this work. At near-physiological conditions (67 mM phosphate buffer, pH 7.4, I=0.17, 37 degrees C), the individual solution of 100 mu M captopril and the co-binding solution with 60 mu M hydrochlorothiazide added were pre-equilibrated with series concentrations of HSA (10-475 mu M) respectively, introducing hydrodynamically into an uncoated fused silica capillary (35 cm x 50 mu m I.D. with 26.5 cm effective length). The values of number of binding sites, the binding constant for captopril and hydrochlorothiazide binding to HSA were obtained, respectively. It can be found that both drugs exhibit moderate binding properties towards HSA and there does not exist significant competitive binding effects between them. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | PLASMA-PROTEIN-BINDING ; HIGH-THROUGHPUT ; DRUG ; SPECTROSCOPY ; COMBINATION ; CONSTANTS ; SITES |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000363439500004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276328]  |
| 专题 | 药物质量控制与固体化学研究中心 药物安全性评价中心 |
| 通讯作者 | Chen, Dong-Ying |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Lab Pharmaceut Anal, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ting-Ting,Xiang, Li-Li,Wang, Jian-Ling,et al. Application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction of captopril with human serum albumin in the absence and presence of hydrochlorothiazide[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2015,115:31-35. |
| APA | Liu, Ting-Ting,Xiang, Li-Li,Wang, Jian-Ling,&Chen, Dong-Ying.(2015).Application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction of captopril with human serum albumin in the absence and presence of hydrochlorothiazide.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,115,31-35. |
| MLA | Liu, Ting-Ting,et al."Application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction of captopril with human serum albumin in the absence and presence of hydrochlorothiazide".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 115(2015):31-35. |
入库方式： OAI收割
来源：上海药物研究所
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