Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy
文献类型：期刊论文
| 作者 | Zhao, Lijuan; Chen, Dongying
|
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2015-01 |
| 卷号 | 29期号:1页码:123-128 |
| 关键词 | capillary electrophoresis frontal analysis circular dichroism drug binding methoxatin disodium salt human serum albumin |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.3248 |
| 文献子类 | Article |
| 英文摘要 | Pressure-assisted capillary electrophoresis (PACE)/frontal analysis (FA) and circular dichroism spectroscopy were utilized to investigate the interactions between methoxatin disodium salt (PQQ-2Na) and human serum albumin (HSA). With the PACE/FA method, sodium phosphate buffer solution (67mm, pH7.4) was used as the background electrolyte. Hydrodynamic injection at 50mbar for 50s and external pressure of 50mbar were applied. The binding constant and the number of primary binding sites to HSA were obtained under fixed concentration of PQQ-2Na (100 mu m) and increasing HSA concentration (0 similar to 475 mu m). The thermodynamic parameters characterized the main acting forces of hydrophobic and electrostatic interactions. The displacement experiments using phenylbutazone and flurbiprofen as ligand markers suggested that the binding site was the Sudlow site I of the HSA molecule. Circular dichroism spectroscopy was further employed to evaluate the conformation changes of HSA under the interaction of PQQ-2Na. This work provides comprehensive information for understanding the interactions between PQQ-2Na and HSA. Copyright (c) 2014 John Wiley & Sons, Ltd. |
| WOS关键词 | PLASMA-PROTEIN-BINDING ; PYRROLOQUINOLINE QUINONE ; FRONTAL ANALYSIS ; DRUG-BINDING ; EQUILIBRIUM DIALYSIS ; HIGH-THROUGHPUT ; IN-VITRO ; CONSTANTS ; FORCES ; SITE |
| 资助项目 | Key New Drug Creation and Manufacturing Program, National Science and Technology Major Project of China[2012ZX09301001-001] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000346908100014 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276776]  |
| 专题 | 药物质量控制与固体化学研究中心 上海药物代谢研究中心 |
| 通讯作者 | Chen, Dongying |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Lab Pharmaceut Anal, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lijuan,Chen, Dongying. Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy[J]. BIOMEDICAL CHROMATOGRAPHY,2015,29(1):123-128. |
| APA | Zhao, Lijuan,&Chen, Dongying.(2015).Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy.BIOMEDICAL CHROMATOGRAPHY,29(1),123-128. |
| MLA | Zhao, Lijuan,et al."Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy".BIOMEDICAL CHROMATOGRAPHY 29.1(2015):123-128. |
入库方式： OAI收割
来源：上海药物研究所
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