Quantitative proteomic analysis reveals the neuroprotective effects of huperzine A for amyloid beta treated neuroblastoma N2a cells
文献类型：期刊论文
| 作者 | Tao, Yimin2,3; Fang, Li2,3; Yang, Yiming2,3; Jiang, Hualiang2 ; Yang, Huaiyu2; Zhang, Haiyan2,3; Zhou, Hu1,2,3
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| 刊名 | PROTEOMICS
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| 出版日期 | 2013-04 |
| 卷号 | 13期号:8页码:1314-1324 |
| 关键词 | Alzheimer's disease Animal proteomics LC-MS MS Huperzine A p53 |
| ISSN号 | 1615-9853 |
| DOI | 10.1002/pmic.201200437 |
| 文献子类 | Article |
| 英文摘要 | Alzheimer's disease is a worldwide metabolic disease and an economically costly disease to society, so more medicines need to be developed to treat this disease. Huperzine A, a novel lycopodium alkaloid isolated from traditional Chinese medicine Huperzia serrata (Qian Ceng Ta), has been shown to possess multiple neuroprotective effects for Alzheimer's disease, but the precise pharmacological mechanism of huperzine A is unclear and needs to be further investigated. In this study, proteins from untreated N2a cells (Con group), cells preincubated with huperzine A followed by A (142) oligomers treatment (HupA group) and cells treated with A (142) oligomers (A group) with five biological replicates in each cohort, were processed in a centrifugal proteomic reactor and quantified by label-free quantitation. A total of 2860 proteins were quantified with high confidence, and 198 proteins were significantly changed (with p-value < 0.05) between HupA and A cohorts. The pathway and direct proteinprotein interaction network analysis showed that huperzine A protects N2a cells against A oligomer-induced cell death by downregulation of cellular tumor antigen p53 (Trp53) expression. |
| WOS关键词 | ATTENUATES MITOCHONDRIAL DYSFUNCTION ; RAT CORTICAL-NEURONS ; ALZHEIMERS-DISEASE ; CHOLINESTERASE INHIBITOR ; THERAPEUTIC TARGETS ; PRION PROTEIN ; EXPRESSION ; IDENTIFICATION ; APOPTOSIS ; PEPTIDE |
| 资助项目 | Chinese Academy of Sciences[00000000] ; E-institutes of Shanghai Municipal Education Commission[E03008] ; Shanghai Science and Technology Development Fund[12QA1404000] ; Ministry of Science and Technology of China[2011CB510004] ; National Natural Science Foundation of China[81173034] ; National Natural Science Foundation of China[81072646] ; Natural Science Foundation of Shanghai[12ZR1436400] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000317684800009 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277671]  |
| 专题 | 药物发现与设计中心 药理学第二研究室 药物安全性评价中心 |
| 通讯作者 | Zhang, Haiyan |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Tao, Yimin,Fang, Li,Yang, Yiming,et al. Quantitative proteomic analysis reveals the neuroprotective effects of huperzine A for amyloid beta treated neuroblastoma N2a cells[J]. PROTEOMICS,2013,13(8):1314-1324. |
| APA | Tao, Yimin.,Fang, Li.,Yang, Yiming.,Jiang, Hualiang.,Yang, Huaiyu.,...&Zhou, Hu.(2013).Quantitative proteomic analysis reveals the neuroprotective effects of huperzine A for amyloid beta treated neuroblastoma N2a cells.PROTEOMICS,13(8),1314-1324. |
| MLA | Tao, Yimin,et al."Quantitative proteomic analysis reveals the neuroprotective effects of huperzine A for amyloid beta treated neuroblastoma N2a cells".PROTEOMICS 13.8(2013):1314-1324. |
入库方式： OAI收割
来源：上海药物研究所
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