Highly Sensitive Liquid Chromatographic Analysis of Artemisinin and Its Derivatives after Pre-Column Derivatization with 4-Carboxyl-2,6-Dinitrobenzene Diazonium Ion
文献类型：期刊论文
| 作者 | Adegoke, O. A.1,2; Xiang, L. L.1; Idowu, O. S.2; Chen, D-Y1 |
| 刊名 | ACTA CHROMATOGRAPHICA
 |
| 出版日期 | 2012-09 |
| 卷号 | 24期号:3页码:445-462 |
| 关键词 | artemisinin derivatives 4-carboxyl-2,6-dinitrobenzene diazonium ion (CDNBD) precolumn derivatization HPLC |
| ISSN号 | 1233-2356 |
| DOI | 10.1556/AChrom.24.2012.3.8 |
| 文献子类 | Article |
| 英文摘要 | A highly sensitive and reproducible isocratic liquid chromatographic method has been developed for the analysis of artemisinin and its three commonly used derivatives (artesunate, dihydroartemisinin, and artemether). The method involves a precolumn derivatization reaction with 4-carboxyl-2,6-dinitrobenzene diazonium ion to produce azo adducts that are UV-active. The critical parameters for the derivatization such as temperature, reaction time, and reagent concentrations were studied and optimized. The chromatographic separations were carried out on a C-18 column with mobile phase consisting of acetonitrile-0.1% acetic acid (60:40) at a flow rate of 1 mL min(-1). UV detection was set at 254 nm. Dynamic linear calibration range was obtained at concentrations of artemisinins ranging from 0.26 to 1.44 mu g mL(-1). The low limits of detections of artemisinin, artesunate, dihydroartemisinin, and artemether were found to be 0.091, 0.0125, 0.0489, and 0.0128 ng mu L-1, respectively. The developed methods were precise (RSD <3%) and accurate (% error <5%). The developed methods may find application in dosage form analysis and pharmacokinetic studies. |
| WOS关键词 | REDUCTIVE ELECTROCHEMICAL DETECTION ; TANDEM MASS-SPECTROMETRY ; HUMAN-PLASMA ; ULTRAVIOLET DETECTION ; ARTEMETHER ; DIHYDROARTEMISININ ; ARTESUNATE ; HPLC ; ANNUA ; LUMEFANTRINE |
| 资助项目 | CAS-TWAS Postdoctoral Fellowship[00000000] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000307557600008 |
| 出版者 | AKADEMIAI KIADO ZRT |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277975]  |
| 专题 | 药物质量控制与固体化学研究中心 |
| 通讯作者 | Adegoke, O. A. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Pharmaceut Anal, Shanghai 201203, Peoples R China 2.Univ Ibadan, Fac Pharm, Dept Pharmaceut Chem, Ibadan, Nigeria; |
| 推荐引用方式 GB/T 7714 | Adegoke, O. A.,Xiang, L. L.,Idowu, O. S.,et al. Highly Sensitive Liquid Chromatographic Analysis of Artemisinin and Its Derivatives after Pre-Column Derivatization with 4-Carboxyl-2,6-Dinitrobenzene Diazonium Ion[J]. ACTA CHROMATOGRAPHICA,2012,24(3):445-462. |
| APA | Adegoke, O. A.,Xiang, L. L.,Idowu, O. S.,&Chen, D-Y.(2012).Highly Sensitive Liquid Chromatographic Analysis of Artemisinin and Its Derivatives after Pre-Column Derivatization with 4-Carboxyl-2,6-Dinitrobenzene Diazonium Ion.ACTA CHROMATOGRAPHICA,24(3),445-462. |
| MLA | Adegoke, O. A.,et al."Highly Sensitive Liquid Chromatographic Analysis of Artemisinin and Its Derivatives after Pre-Column Derivatization with 4-Carboxyl-2,6-Dinitrobenzene Diazonium Ion".ACTA CHROMATOGRAPHICA 24.3(2012):445-462. |
入库方式： OAI收割
来源：上海药物研究所
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