Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation
文献类型：期刊论文
| 作者 | Peng, Nanqiu1; Zhong, Yi1; Zhang, Qing1 ; Zheng, Mingyue7; Zhao, Wei1,4,5,6; Jiang, Hualiang7; Yang, Chen1; Guo, Xiaokui3; Zhao, Guoping1,2,5,6
|
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2012-08 |
| 卷号 | 44期号:8页码:692-702 |
| 关键词 | acetyl-CoA carboxylase propionyl-CoA carboxylase Leptospira interrogans CO2 assimilation |
| ISSN号 | 1672-9145 |
| DOI | 10.1093/abbs/gms047 |
| 文献子类 | Article |
| 英文摘要 | Leptospira interrogans is the causative agent of leptospirosis. The in vitro growth of L. interrogans requires CO2 and a partial 3-hydroxypropionate pathway involving two acyl-CoA carboxylases was suggested by genomic analysis to assimilate CO2. Either set of the candidate genes heterologously co-expressed in Escherichia coli was able to demonstrate both acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC) activities. The tri-subunit holoenzyme (LA_2736-LA_2735 and LA_3803), although failed to be purified, was designated ACC based on its substrate preference toward acetyl-CoA. The partially purified bi-subunit holoenzyme (LA_2432-LA_2433) has a considerably higher activity against propionyl-CoA as the substrate than that of acetyl-CoA, and thus, designated PCC. Native polyacrylamide gel electrophoresis indicated that this PCC has a molecular mass of around 669 kDa, suggesting an (44) quaternary structure and both structural homology modeling and site-directed mutagenesis analysis of its carboxyltransferase subunit (LA_2433) indicated that the A431 residue located at the bottom of the putative substrate binding pocket may play an important role in substrate specificity determination. Both transcriptomic and proteomic data indicated that enzymes involved in the suggested partial 3-hydroxypropionate pathway were expressed in vivo in addition to ACC/PCC and the homologous genes in genomes of other Leptospira species were re-annotated accordingly. However, as the in vitro detected specific activity of ACC in the crude cell extract was too low to account for the growth of the bacterium in EllinghausenMcCulloughJohnsonHarris minimal medium, further systematic analysis is required to unveil the mechanism of gluconeogenesis via anaplerotic CO2 assimilation in Leptospira species. |
| WOS关键词 | COENZYME-A CARBOXYLASE ; STREPTOMYCES-COELICOLOR A3(2) ; AUTOTROPHIC CARBON FIXATION ; OUTER-MEMBRANE PROTEIN ; 3-HYDROXYPROPIONATE CYCLE ; PATHOGENIC LEPTOSPIRAE ; SUBSTRATE-SPECIFICITY ; CITRAMALATE SYNTHASE ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI |
| 资助项目 | National Natural Science Foundation of China[30830002] ; National Natural Science Foundation of China[31121001] ; National Basic Research Program of China[2012CB721102] ; Research Unit Fund of Li Ka Shing Institute of Health Sciences[7103506] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4636027 |
| WOS记录号 | WOS:000306922800009 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278002]  |
| 专题 | 药物发现与设计中心 |
| 通讯作者 | Zhao, Guoping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China; 2.Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Dept Microbiol, Shatin, Hong Kong, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Dept Microbiol & Parasitol, Shanghai 200025, Peoples R China; 4.China HKY Gene Technol Co Ltd, Shenzhen 518057, Guangdong, Peoples R China; 5.Fudan Univ, Sch Life Sci, Key Lab Med Mol Virol, Minist Hlth,Shanghai Med Coll,Dept Microbiol, Shanghai 200433, Peoples R China; 6.Fudan Univ, Sch Life Sci, Key Lab Med Mol Virol, Minist Educ,Shanghai Med Coll,Dept Microbiol, Shanghai 200433, Peoples R China; 7.Chinese Acad Sci, Shanghai Inst Mat Med, DDDC, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Peng, Nanqiu,Zhong, Yi,Zhang, Qing,et al. Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(8):692-702. |
| APA | Peng, Nanqiu.,Zhong, Yi.,Zhang, Qing.,Zheng, Mingyue.,Zhao, Wei.,...&Zhao, Guoping.(2012).Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(8),692-702. |
| MLA | Peng, Nanqiu,et al."Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.8(2012):692-702. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。