Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro
文献类型：期刊论文
| 作者 | Liu, Fei1; Chen, Yang1; Yang, Bing1; Wang, Jingfang3; Peng, Qin1; Shao, Qimiao1; Li, Xuan1; Beerntsen, Brenda T.2; Xu, Yechun6 ; Li, Jianyong5
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2012-03 |
| 卷号 | 36期号:3页码:619-628 |
| 关键词 | Drosophila melanogaster Prophenoloxidases Expression Cu2+ association Activation |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2011.12.001 |
| 文献子类 | Article |
| 英文摘要 | Dipteran insects, like mosquitoes, possess more than two prophenoloxidase (PPO) genes, but it is unclear whether their gene products differ in biochemical properties and physiological functions. Here, we used three Drosophila melanogaster PPOs as models to study their properties through expression in S2 cells. Our data revealed that the PPOs were expressed in the ethanol-activatable conformation: rPPO1 and rPPO2 needed additional Cu2+ in the medium, but rPPO3 did not. rPPO1 bound Cu2+ within minutes; rPPO2 did that in hours when Cu2+ were present at a higher concentration. Thus, rPPO1 and rPPO2 were expressed as apo-rPPO and became holo-PPO upon Cu2+ binding; rPPO3 was holo-PPO immediately after expression. Surprisingly, in the absence of ethanol, the apparently intact rPPO3 catalyzed dopamine oxidation and melanization. The successful method for rPPO expression in S2 cells described in this paper will provide us with an opportunity to study the properties of a specific PPO gene in a small insect like mosquitoes in the future. (c) 2011 Elsevier Ltd. All rights reserved. |
| WOS关键词 | MANDUCA-SEXTA ; PHENOLOXIDASE ACTIVITY ; IMMUNE-RESPONSES ; ACTIVATION ; INSECT ; MELANIZATION ; 2-PROPANOL ; HEMOLYMPH ; A(1) |
| 资助项目 | National Natural Science Foundation of China[30970408] ; National Natural Science Foundation of China[31172151] ; Chinese Academy of Sciences[KSCX2-EW-J-12] ; Chinese Academy of Sciences[20090HTP05] ; Ministry of Agriculture of China[2008ZX08009-003] |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000301170800015 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278172]  |
| 专题 | 药物发现与设计中心 |
| 通讯作者 | Ling, Erjun |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Insect Dev & Evolutionary Biol, Shanghai 200032, Peoples R China; 2.Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA; 3.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, Shanghai 200240, Peoples R China; 4.Univ Missouri, Sch Biol Sci, Div Cell Biol & Biophys, Kansas City, MO 64110 USA 5.Virginia Polytech Inst & State Univ, Dept Biochem, Blacksburg, VA 24061 USA; 6.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Discovery & Design, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, Fei,Chen, Yang,Yang, Bing,et al. Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,36(3):619-628. |
| APA | Liu, Fei.,Chen, Yang.,Yang, Bing.,Wang, Jingfang.,Peng, Qin.,...&Ling, Erjun.(2012).Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,36(3),619-628. |
| MLA | Liu, Fei,et al."Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 36.3(2012):619-628. |
入库方式： OAI收割
来源：上海药物研究所
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