Application of phase optimized liquid chromatography to oligopeptide separations
文献类型：期刊论文
| 作者 | Lu, Jing2; Ji, Min2; Ludewig, Ronny1; Scriba, Gerhard K. E.1; Chen, Dong-ying2
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2010-02-05 |
| 卷号 | 51期号:3页码:764-767 |
| 关键词 | Phase optimized liquid chromatography POPLC Oligopeptide HPLC Gradient elution |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2009.09.036 |
| 文献子类 | Article |
| 英文摘要 | Stationary phase optimized liquid chromatography (POPLC) has been applied to the separation of oligopeptides. The retention factors and theoretical plate numbers of 13 peptides were determined on five different stationary phases. Based on these values, an optimal stationary phase composition of 250 mm total length consisting of 3 segments of 20 mm octadecyl silica, 10 mm phenyl silica and 220 mm embedded polar octadecyl silica was calculated by the optimizer software. Good agreement between the calculated and experimental chromatograms was observed. In order to achieve short analysis time and baseline separation of all peptides gradient elution and optimization of the column temperature were performed. The optimized mobile phase conditions consisted of (A) acetonitrile and (B) 0.025 M aqueous sodium phosphate buffer, pH 3.0, operated at 10% A (0-12 min) followed by 10-50% A (12-32 min) at a flow rate of 0.5 mL min(-1) and column temperature of 35 degrees C. Using these conditions all peptides could be separated in less than 30 min with good resolution and peak symmetry. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS关键词 | HPLC |
| 资助项目 | Sino-German Center for Research Promotion[00000000] ; DFG[00000000] ; NSFC[GZ364] ; National Science & Technology Major Project of China[2009ZX09301-001] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000272025800038 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278974]  |
| 专题 | 药物质量控制与固体化学研究中心 药物安全性评价中心 |
| 通讯作者 | Chen, Dong-ying |
| 作者单位 | 1.Univ Jena, Dept Med Pharmaceut Chem, D-07743 Jena, Germany 2.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Pharmaceut Anal, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lu, Jing,Ji, Min,Ludewig, Ronny,et al. Application of phase optimized liquid chromatography to oligopeptide separations[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2010,51(3):764-767. |
| APA | Lu, Jing,Ji, Min,Ludewig, Ronny,Scriba, Gerhard K. E.,&Chen, Dong-ying.(2010).Application of phase optimized liquid chromatography to oligopeptide separations.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,51(3),764-767. |
| MLA | Lu, Jing,et al."Application of phase optimized liquid chromatography to oligopeptide separations".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 51.3(2010):764-767. |
入库方式： OAI收割
来源：上海药物研究所
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