Application of headspace solid phase microextraction for study of noncovalent interaction of borneol with human serum albumin
文献类型：期刊论文
| 作者 | Hu, Liang; Chen, Dong-ying
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2009-11 |
| 卷号 | 30期号:11页码:1573-1576 |
| 关键词 | borneol human serum albumin binding gas chromatography headspace solid phase microextraction |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2009.148 |
| 文献子类 | Article |
| 英文摘要 | Aim: To investigate noncovalent interactions between borneol and human serum albumin (HSA) under near-physiological conditions. Methods: A 65-mu m polydimethylsiloxane ( PDMS) fiber was selected for sampling. The extraction temperature was kept at 37 degrees C, and the extraction time was optimized at 10 min. Borneol solutions of different concentrations were equilibrated in 600 mu mol/L HSA and 67 mmol/L phosphate buffer solution (pH 7.4, 37 degrees C) for 24 h prior to solid phase microextraction (SPME) using headspace mode. The binding properties were obtained based on the calculation of extracted borneol amount using gas chromatography ( GC) determination. Results: The headspace SPME extraction method avoided disturbance from the HSA binding matrix. The recovery showed good linearity for the borneol concentrations over the range of 0.4-16.3 mu mol/L with a regression coefficient (R-2) of 0.9998. The limit of detection and lower limit of quantitation were determined to be 0.01 mu mol/L and 0.4 mu mol/L, respectively. The binding constant and the percentage binding rate were estimated to be 2.4x10(3) (mol/L)(-1) and 59.5%, respectively. Conclusion: Headspace SPME coupled to GC is a simple, sensitive and rapid method for the study of borneol binding to HSA. The method may be applied in the determination of other protein binding properties in human plasma. |
| WOS关键词 | BINDING ; PROTEIN ; DRUGS ; MATRICES ; SPME |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3758776 |
| WOS记录号 | WOS:000271953500013 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279085]  |
| 专题 | 药物质量控制与固体化学研究中心 |
| 通讯作者 | Chen, Dong-ying |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Liang,Chen, Dong-ying. Application of headspace solid phase microextraction for study of noncovalent interaction of borneol with human serum albumin[J]. ACTA PHARMACOLOGICA SINICA,2009,30(11):1573-1576. |
| APA | Hu, Liang,&Chen, Dong-ying.(2009).Application of headspace solid phase microextraction for study of noncovalent interaction of borneol with human serum albumin.ACTA PHARMACOLOGICA SINICA,30(11),1573-1576. |
| MLA | Hu, Liang,et al."Application of headspace solid phase microextraction for study of noncovalent interaction of borneol with human serum albumin".ACTA PHARMACOLOGICA SINICA 30.11(2009):1573-1576. |
入库方式： OAI收割
来源：上海药物研究所
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