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A New Artemisinin Derivative SM1044 Induces Apoptosis of Kasumi-1 Cells and Its Mechanism

文献类型：期刊论文

作者Liu Jingjing1; Fei Aimei1; Nie Ruimin1; Wang Jin1; Li Ying2; Wang Zhenyi1; Mi Jianqing1
刊名Journal of Experimental Hematology
出版日期2011
卷号19期号:3页码:607-611
关键词SM1044 Artemisinin derivative SM1044 Kasumi-1 cell AML1-ETO fusion gene cell apoptsis
ISSN号1009-2137
其他题名新型青蒿素衍生物SM1044诱导Kasumi-1细胞凋亡及机制的研究
文献子类Article
英文摘要The aim of this study was to investigate the apoptosis-inducing effect of artemisinin derivative SM1044 on Kasumi-1 cells and its possible mechanism. Kasumi-1 cells were treated with different concentrations of SM1044, the cell viability was evaluated by MTT assay. Cell apoptosis and cell cycle progression were assessed by using flow cytometry with Annexin-V/PI double staining and flow cytometry with PI staining respectively. The expression of apoptosis-related proteins caspase 3, PARP and the fusion protein AML1-ETO were detected by Western blot. The results indicated that SM1044 inhibited cell growth of Kasumi-1 cells in time-and dose-dependent manners. After exposure of Kasumi-1 cells to 1mumol/L SM1044 for 24 hours, the cell viability was decreased to 50%. IC_(50) of SM1044 to Kasumi-1 cells at 48 hours was 0.170.067mumol/L. SM1044 induced cell apoptosis in a caspase-dependent manner, and the apoptotic rate of Kasumi-1 cells increased as SM1044 concentration increased. Flow cytometry with PI staining revealed that SM1044 induced cell cycle arrest, and the proportion of cells in G_0/G_1 phase increased from 58.334.46% to 71.752.24% after exposure to 5mumol/L SM1044 for 24 hours. Western blot showed that SM1044 increased the expression of apoptosis-related proteins cPARP and cleaved caspase 3 and also degraded the AML1-ETO fusion protein. It is concluded that SM1044 can inhibit the proliferation of Kasumi-1 cells, induce cell apoptosis which may be releted to the increased level of cleaved PARP and cleaved caspase 3. SM1044 can also induce cell arrest in G_0/G_1 phase. As the fusion protein AML1-ETO degrades obviously, it can be the potential target of SM1044 in Kasumi-1 cells.
资助项目国家自然科学基金面上项目[00000000] ; 973国家重大基础研究发展计划[00000000]
WOS研究方向Oncology ; Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:4344688
源URL[http://119.78.100.183/handle/2S10ELR8/267902]  
专题药物化学研究室
作者单位1.State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Shanghai 200025, China.;
2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式
GB/T 7714		 Liu Jingjing,Fei Aimei,Nie Ruimin,et al. A New Artemisinin Derivative SM1044 Induces Apoptosis of Kasumi-1 Cells and Its Mechanism[J]. Journal of Experimental Hematology,2011,19(3):607-611.
APA Liu Jingjing.,Fei Aimei.,Nie Ruimin.,Wang Jin.,Li Ying.,...&Mi Jianqing.(2011).A New Artemisinin Derivative SM1044 Induces Apoptosis of Kasumi-1 Cells and Its Mechanism.Journal of Experimental Hematology,19(3),607-611.
MLA Liu Jingjing,et al."A New Artemisinin Derivative SM1044 Induces Apoptosis of Kasumi-1 Cells and Its Mechanism".Journal of Experimental Hematology 19.3(2011):607-611.

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来源：上海药物研究所

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