White matter injury after cardiopulmonary bypass in a brain slice model of neonatal rats with perfusion
文献类型：期刊论文
| 作者 | Huang Junrong1; Zhu Zhongqun1; Liu Gang1; Chen Huiwen1; Feng Linyin2
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| 刊名 | Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
 |
| 出版日期 | 2017 |
| 卷号 | 24期号:4页码:295-300 |
| 关键词 | Congenital heart disease cardiopulmonary bypass deep hypothermic cardiac arrest white matter preoligodendrocytes |
| ISSN号 | 1007-4848 |
| 其他题名 | 新生小鼠脑切片体外循环灌流模型中白质损伤的研究 |
| 文献子类 | Article |
| 英文摘要 | Objective Through establishment of brain slice model in rats with perfusion and oxygen glucose deprivation (OGD), we investigated whether this model can replicate the pathophysiology of brain injury in cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA) or not and whether perfusion and OGD can induce preoligodendrocytes (preOL) injury or not, to provide cytological evidence for white matter injury after cardiopulmonary bypass. Methods Three to five living brain slices were randomly obtained from each of forty sevenday- old (P7) Sprague-Dawley (SD) rats with a mean weight of 14.71.5 g. Brain slices were randomly divided into five groups with 24 slices in each group: control group with normothermic artificial cerebralspinal fluid (aCSF) perfusion(36℃) and DHCA groups: OGD at 15℃, 25℃, 32℃ and 36℃. The perfusion system was established, and the whole process of CPB and DHCA in cardiac surgery was simulated. The degree of oligodendrocyte injury was evaluated by MBP and O4 antibody via application of immunohistochemistry. Results In the OGD group, the mature oligodendrocytes(MBP-positive) cells were significantly damaged, their morphology was greatly changed and fluorescence expression was significantly reduced. The higher the OGD temperature was, the more serious the damage was; preOL (O4-positive) cells showed different levels of fluorescence expression reduce in 36℃, 32℃ and 25℃ groups, and the higher the OGD temperature was, the more obvious decrease in fluorescence expression was. There was no statistically significant difference in the 04-positive cells between the control group and the 15°C OGD group. Conclusion The perfused brain slice model is effective to replicate the pathophysiology of brain injury in CPB/DHCA which can induce preOL damage that is in critical development stages of oligodendrocyte cell line, and reduce differentiation of oligodendrocyte cells and eventually leads to hypomyelination as well as cerebral white matter injury. |
| WOS研究方向 | General & Internal Medicine ; Surgery (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5957334 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269209]  |
| 专题 | 药理学第二研究室 |
| 作者单位 | 1.Department of Cardiothoracic Surgery,Shanghai Children's Medical Center,Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.; 2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Huang Junrong,Zhu Zhongqun,Liu Gang,et al. White matter injury after cardiopulmonary bypass in a brain slice model of neonatal rats with perfusion[J]. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery,2017,24(4):295-300. |
| APA | Huang Junrong,Zhu Zhongqun,Liu Gang,Chen Huiwen,&Feng Linyin.(2017).White matter injury after cardiopulmonary bypass in a brain slice model of neonatal rats with perfusion.Chinese Journal of Clinical Thoracic and Cardiovascular Surgery,24(4),295-300. |
| MLA | Huang Junrong,et al."White matter injury after cardiopulmonary bypass in a brain slice model of neonatal rats with perfusion".Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 24.4(2017):295-300. |
入库方式： OAI收割
来源：上海药物研究所
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