Discovery of a Potential Plasma Protein Biomarker Panel for Acute-on-Chronic Liver Failure Induced by Hepatitis B Virus
文献类型：期刊论文
| 作者 | Zhou, Ni2; Wang, Kuifeng2; Fang, Shanhua1,3,4; Zhao, Xiaoyu2; Huang, Tingting2; Chen, Huazhong2; Yan, Fei2; Tang, Yongzhi2; Zhou, Hu1,3,4 ; Zhu, Jiansheng2
|
| 刊名 | FRONTIERS IN PHYSIOLOGY
 |
| 出版日期 | 2017-12-06 |
| 卷号 | 8 |
| ISSN号 | 1664-042X |
| 关键词 | HBV-ACLF CHB iTRAQ proteomics biomarker |
| DOI | 10.3389/fphys.2017.01009 |
| 文献子类 | Article |
| 英文摘要 | Hepatitis B virus (HBV)-associated acute-on-chronic liver failure (HBV-ACLF), characterized by an acute deterioration of liver function in the patients with chronic hepatitis B (CHB), is lack of predicting biomarkers for prognosis. Plasma is an ideal sample for biomarker discovery due to inexpensive and minimally invasive sampling and good reproducibility. In this study, immuno-depletion of high-abundance plasma proteins followed by iTRAQ-based quantitative proteomic approach was employed to analyze plasma samples from 20 healthy control people, 20 CHB patients and 20 HBV-ACLF patients, respectively. As a result, a total of 427 proteins were identified from these samples, and 42 proteins were differentially expressed in HBV-ACLF patients as compared to both CHB patients and healthy controls. According to bioinformatics analysis results, 6 proteins related to immune response (MMR), inflammatory response (OPN, HPX), blood coagulation (ATIII) and lipid metabolism (APO-CII, GP73) were selected as biomarker candidates. Further ELISA analysis confirmed the significant up-regulation of GP73, MMR, OPN and down-regulation of ATIII, HPX, APO-CII in HBV-ACLF plasma samples (p < 0.01). Moreover, receiver operating characteristic (ROC) curve analysis revealed high diagnostic value of these candidates in assessing HBV-ACLF. In conclusion, present quantitative proteomic study identified 6 novel HBV-ACLF biomarker candidates and might provide fundamental information for development of HBV-ACLF biomarker. |
| WOS关键词 | SERUM PROTEOMIC ANALYSIS ; HEPATOCELLULAR-CARCINOMA ; MANNOSE RECEPTOR ; LIPOPROTEIN METABOLISM ; MASS-SPECTROMETRY ; OSTEOPONTIN ; DISEASE ; EXPRESSION ; HEMOPEXIN ; GP73 |
| 资助项目 | Zhejiang Province Major Science and Technology Programs[2012C13018-3] ; Zhejiang Provincial Natural Science Foundation[LY15H030003] ; National Natural Science Foundation of China[21375138] ; National Natural Science Foundation of China[81500469] ; Chinese Academy of Science[XDA12030203] |
| WOS研究方向 | Physiology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000417156700001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272358]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Zhou, Hu; Zhu, Jiansheng |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China 2.Wenzhou Med Univ, Dept Infect Dis, Affiliated Taizhou Hosp, Taizhou, Peoples R China; 3.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, Shanghai, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhou, Ni,Wang, Kuifeng,Fang, Shanhua,et al. Discovery of a Potential Plasma Protein Biomarker Panel for Acute-on-Chronic Liver Failure Induced by Hepatitis B Virus[J]. FRONTIERS IN PHYSIOLOGY,2017,8. |
| APA | Zhou, Ni.,Wang, Kuifeng.,Fang, Shanhua.,Zhao, Xiaoyu.,Huang, Tingting.,...&Zhu, Jiansheng.(2017).Discovery of a Potential Plasma Protein Biomarker Panel for Acute-on-Chronic Liver Failure Induced by Hepatitis B Virus.FRONTIERS IN PHYSIOLOGY,8. |
| MLA | Zhou, Ni,et al."Discovery of a Potential Plasma Protein Biomarker Panel for Acute-on-Chronic Liver Failure Induced by Hepatitis B Virus".FRONTIERS IN PHYSIOLOGY 8(2017). |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。