Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells
文献类型：期刊论文
| 作者 | Pan, Duyi2; Gao, Jing1,4; Zeng, Xiaoqing2; Ma, Guifen5; Li, Na2; Huang, Xiaoquan2; Du, Xuanling2; Miao, Qing2; Lian, Jingjing2; Xu, Lili2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-10-31 |
| 卷号 | 7 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-017-14453-2 |
| 文献子类 | Article |
| 英文摘要 | Forkhead box protein 3 (FOXP3) is implicated in tumor progression and prognosis in various types of tumor cells. We have recently reported that FOXP3 inhibited proliferation of gastric cancer (GC) cells through activating the apoptotic signaling pathway. In this study, we found that over-expression of FOXP3 inhibited GC cell migration, invasion and proliferation. Then, the label-free quantitative proteomic approach was employed to further investigating the down-stream proteins regulated by FOXP3, resulting in a total of 3,978 proteins quantified, including 186 significantly changed proteins. Caveolin-1 (CAV1), as a main constituent protein of caveolae, was one of those changed proteins upregulated in FOXP3-overexpressed GC cells, moreover, it was assigned as one of the node proteins in the protein-protein interaction network and the key protein involved in focal adhesion pathway by bioinformatics analysis. Further biological experiments confirmed that FOXP3 directly bound to the promoter regions of CAV1 to positively regulate CAV1 transcription in GC cells. In summary, our study suggested that FOXP3 can be considered as a tumor suppressor in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation axis may play an important role in inhibiting invasion and metastasis of GC cells. Data are available via ProteomeXchange under identifier PXD007725. |
| WOS关键词 | EARLY TUMOR RECURRENCE ; PROSTATE-CANCER ; DOWN-REGULATION ; BREAST-CANCER ; COMPUTATIONAL PLATFORM ; INTERACTION NETWORKS ; COLORECTAL-CANCER ; SUPPRESSOR GENE ; T-CELLS ; EXPRESSION |
| 资助项目 | Natural Science Foundation of Shanghai[14ZR1406600] ; National Natural Science Foundation of China[21375138] ; Ministry of Science and Technology of China[2013ZX09507001] ; "one hundred talent program" of Chinese Academy of Sciences[00000000] ; Strategic Priority Research Program of the Chinese Academy of Sciences, "Personalized Medicines-Molecular Signature-based Drug Discovery and Development"[XDA12030203] ; Innovation Project of Instrument and Equipment Function Development from the Bureau of Goods, Chinese Academy of Sciences[YZ201542] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000414231000061 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272437]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Zhou, Hu; Chen, Shiyao |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai, Peoples R China; 2.Fudan Univ, Zhongshan Hosp, Dept Gastroenterol, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China; 5.Fudan Univ, Zhongshan Hosp, Dept Radiotherapy, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Pan, Duyi,Gao, Jing,Zeng, Xiaoqing,et al. Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Pan, Duyi.,Gao, Jing.,Zeng, Xiaoqing.,Ma, Guifen.,Li, Na.,...&Chen, Shiyao.(2017).Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells.SCIENTIFIC REPORTS,7. |
| MLA | Pan, Duyi,et al."Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：上海药物研究所
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