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Identification of Intracellular beta-Barrel Residues Involved in Ion Selectivity in the Mechanosensitive Channel of Thermoanaerobacter tengcongensis

文献类型：期刊论文

作者Song, Yingcai3; Zhang, Bing2,3; Guo, Fei2; Yang, Maojun1; Li, Yang2; Liu, Zhi-Qiang3
刊名FRONTIERS IN PHYSIOLOGY
出版日期2017-10-25
卷号8
关键词TtMscS anion selectivity beta-barrel selective filter patch-clamp single mutation
ISSN号1664-042X
DOI10.3389/fphys.2017.00832
文献子类Article
英文摘要The mechanosensitive channel of small conductance (MscS) is a bacterial membrane pore that senses membrane tension and protects cells from lysis by releasing osmolytes. MscS is a homoheptameric channel with a cytoplasmic domain with seven portals and a beta-barrel opening to the cytoplasm. TtMscS, an MscS channel from Thermoanaerobacter tengcongensis, is an anion-selective channel. A previous study from our laboratory has defined the crucial role of beta-barrel in the anion selectivity of TtMscS (Zhang et al., 2012). However, the mechanistic details by which the beta-barrel determines anion selectivity remain unclear. Here, using mutagenesis and patch-clamp recordings, we investigated the function and structural correlations between beta-barrels and the anion selectivity of TtMscS at the atomic level. Our results indicated that mutation of V274, a residue at the center of the inner wall of the beta-barrel in TtMscS, caused the anion selectivity of TtMscS reverse to cation selectivity. Moreover, the electrostatic potential (T272) and physical size (L276) of residues in the inner wall of beta-barrel also determine the anion selectivity of TtMscS. In summary, the present study confirmed that the beta-barrel region of TtMscS acts as a "selective filter" that renders TtMscS anion selectivity.
WOS关键词ESCHERICHIA-COLI ; CHLORIDE CHANNELS ; SMALL-CONDUCTANCE ; MSCS ; FILTER ; TRANSPORTERS ; MECHANISM ; MEMBRANE ; MICROBES ; RECEPTOR
资助项目National Natural Science Foundation[31200771] ; National Natural Science Foundation[31171011] ; National Natural Science Foundation[31371066] ; National Natural Science Foundation[31671049] ; Ministry of Science and Technology[2013CB91060101] ; Science and Technology Commission of Shanghai Municipality[16411967400] ; "Personalized Medicines-Molecular Signature-based Drug Discovery and Development," Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12040214]
WOS研究方向Physiology
语种英语
WOS记录号WOS:000413631900002
出版者FRONTIERS MEDIA SA
源URL[http://119.78.100.183/handle/2S10ELR8/272440]  
专题药理学第二研究室
通讯作者Yang, Maojun; Li, Yang; Liu, Zhi-Qiang
作者单位1.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, Key Lab Prot Sci,Minist Educ, Beijing, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai, Peoples R China;
3.Tongji Univ, Sch Med, Shanghai Matern & Infant Hosp 1, Dept Anaesthesiol, Shanghai, Peoples R China;
推荐引用方式
GB/T 7714		 Song, Yingcai,Zhang, Bing,Guo, Fei,et al. Identification of Intracellular beta-Barrel Residues Involved in Ion Selectivity in the Mechanosensitive Channel of Thermoanaerobacter tengcongensis[J]. FRONTIERS IN PHYSIOLOGY,2017,8.
APA Song, Yingcai,Zhang, Bing,Guo, Fei,Yang, Maojun,Li, Yang,&Liu, Zhi-Qiang.(2017).Identification of Intracellular beta-Barrel Residues Involved in Ion Selectivity in the Mechanosensitive Channel of Thermoanaerobacter tengcongensis.FRONTIERS IN PHYSIOLOGY,8.
MLA Song, Yingcai,et al."Identification of Intracellular beta-Barrel Residues Involved in Ion Selectivity in the Mechanosensitive Channel of Thermoanaerobacter tengcongensis".FRONTIERS IN PHYSIOLOGY 8(2017).

入库方式： OAI收割

来源：上海药物研究所

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