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SYVN1, an ERAD E3 Ubiquitin Ligase, Is Involved in GABA(A)alpha 1 Degradation Associated with Methamphetamine-Induced Conditioned Place Preference

文献类型：期刊论文

作者Jiao, Dong-Liang3,7; Chen, Yan4,5; Liu, Yao7; Ju, Yun-Yue7; Long, Jian-Dong1,6; Du, Jiang7; Yu, Chang-Xi4; Wang, Yu-Jun1,6; Zhao, Min2,7; Liu, Jing-Gen1,6
刊名FRONTIERS IN MOLECULAR NEUROSCIENCE
出版日期2017-10-05
卷号10
ISSN号1662-5099
关键词methamphetamine GABA(A)alpha 1 dorsal striatum ERAD SYVN1
DOI10.3389/fnmol.2017.00313
文献子类Article
英文摘要Abuse of methamphetamine (METH), a powerful addictive amphetamine-type stimulants (ATS), is becoming a global public health problem. The gamma-aminobutyric acid (GABA)ergic system plays a critical role in METH use disorders. By using rat METH conditioned place preference (CPP) model, we previously demonstrated that METH-associated rewarding memory formation was associated with the reduction of GABA(A)alpha 1 expression in the dorsal straitum (Dstr), however, the underlying mechanism was unclear. In the present study, we found that METH-induced CPP formation was accompanied by a significant increase in the expression of Synovial apoptosis inhibitor 1 (SYVN1), an endoplasmic reticulum (ER)-associated degradation (ERAD) E3 ubiquitin ligase, in the Dstr. The siRNA knockdown of SYVN1 significantly increased GABA(A)alpha 1 protein levels in both primary cultured neurons and rodent Dstr. Inhibition of proteasomal activity by MG132 and Lactacystin significantly increased GABA(A)alpha 1 protein levels. We further found that SYVN1 knockdown increased GABA(A)alpha 1 in the intra-ER, but not in the extra-ER. Accordingly, endoplasmic reticulum stress (ERS)-associated Glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) increased. Thus, this study revealed that SYVN1, as the ERAD E3 ubiquitin ligase, was associated with Dstr GABA(A)alpha 1 degradation induced by METH conditioned pairing.
WOS关键词ENDOPLASMIC-RETICULUM STRESS ; UNFOLDED PROTEIN RESPONSE ; RAT-BRAIN ; STIMULANT PSYCHOSIS ; DRUG-ADDICTION ; RECEPTORS ; AMPHETAMINE ; EXPRESSION ; MUTATION ; ALPRAZOLAM
资助项目National Natural Science Foundation of China[U1502228] ; National Natural Science Foundation of China[81771436] ; National Natural Science Foundation of China[81401107] ; National Key R&D Program of China[2017YFC1310400] ; Shanghai Municipal Health and Family Planning Commission[2014ZYJB0002] ; Committee of Science and Technology of Shanghai[13JC140680] ; Shanghai Key Laboratory of Psychotic Disorders[13DZ2260500] ; Nanjing Medical University[2016NJMUZD080] ; Youth Innovation Promotion of the Chinese Academy of Sciences[2017334]
WOS研究方向Neurosciences & Neurology
语种英语
出版者FRONTIERS MEDIA SA
WOS记录号WOS:000412324500001
源URL[http://119.78.100.183/handle/2S10ELR8/272457]  
专题药理学第二研究室
通讯作者Yu, Chang-Xi; Wang, Yu-Jun; Zhao, Min
作者单位1.Chinese Acad Sci, Collaborat Innovat Ctr Brain Sci, Shanghai, Peoples R China;
2.Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Psychot Disorders, Shanghai Mental Hlth Ctr, Shanghai, Peoples R China
3.Bengbu Med Coll, Dept Psychiat, Bengbu, Peoples R China;
4.Fujian Med Univ, Dept Pharmacol, Coll Pharm, Fuzhou, Fujian, Peoples R China;
5.Fujian Univ Tradit Chinese Med, Coll Pharm, Fuzhou, Fujian, Peoples R China;
6.Chinese Acad Sci, Key Lab Receptor Res, Shanghai Inst Mat Med, Shanghai, Peoples R China;
7.Shanghai Jiao Tong Univ, Sch Med, Collaborat Innovat Ctr Brain Sci, Shanghai Mental Hlth Ctr, Shanghai, Peoples R China;
推荐引用方式
GB/T 7714		 Jiao, Dong-Liang,Chen, Yan,Liu, Yao,et al. SYVN1, an ERAD E3 Ubiquitin Ligase, Is Involved in GABA(A)alpha 1 Degradation Associated with Methamphetamine-Induced Conditioned Place Preference[J]. FRONTIERS IN MOLECULAR NEUROSCIENCE,2017,10.
APA Jiao, Dong-Liang.,Chen, Yan.,Liu, Yao.,Ju, Yun-Yue.,Long, Jian-Dong.,...&Liu, Jing-Gen.(2017).SYVN1, an ERAD E3 Ubiquitin Ligase, Is Involved in GABA(A)alpha 1 Degradation Associated with Methamphetamine-Induced Conditioned Place Preference.FRONTIERS IN MOLECULAR NEUROSCIENCE,10.
MLA Jiao, Dong-Liang,et al."SYVN1, an ERAD E3 Ubiquitin Ligase, Is Involved in GABA(A)alpha 1 Degradation Associated with Methamphetamine-Induced Conditioned Place Preference".FRONTIERS IN MOLECULAR NEUROSCIENCE 10(2017).

入库方式： OAI收割

来源：上海药物研究所

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