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Comparative Evaluation of Small Molecular Additives and Their Effects on Peptide/Protein Identification

文献类型：期刊论文

作者Gao, Jing2,3,6; Zhong, Shaoyun2,3,6; Zhou, Yanting1,2,6; He, Han2,6; Peng, Shuying4; Zhu, Zhenyun2,6; Liu, Xing2,6; Zheng, Jing1; Xu, Bin3; Zhou, Hu2,5,6,7
刊名ANALYTICAL CHEMISTRY
出版日期2017-06-06
卷号89期号:11页码:5785-5793
ISSN号0003-2700
DOI10.1021/acs.analchem.6b04886
文献子类Article
英文摘要Detergents and salts are widely used in lysis buffers to enhance protein extraction from biological. samples, facilitating in-depth proteomic analysis., However, these detergents and Salt additives must be efficiently removed from the digested samples prior to LC-MS/MS analysis to obtain high-quality mass spectra. Although filter-aided sample preparation (FASP), acetone precipitation (AP), followed by in-solution digestion,, and strong cation exchange-based centrifugal proteomic reactors (CPRS) are commonly used for proteomic sample processing, little is-known about their efficiencies at,removing detergents and salt additives. In this study, an integrative workflow for the quantification of small molecular additives in proteomic. sample's, developing a multiple reaction monitoring (MRM)-based LC-MS approach for the quantification of six additives (i.e., Tris, urea, CHAPS, SDS, SDC; and Triton X-100) and (ii) systematically evaluated the relationships between the level of additive remaining: in samples following sample processing and the number of peptides/proteins identified by mass Spectrometry. Although FASP outperformed the-other two methods, the results were complementary in terms of peptide/protein identification, as well as the GRAVY index and amino acid distributions. This is the first systematic and quantitative Study-of the effect of detergents and salt, additives on protein identification. This MRM-based approach can be used for an unbiased evaltation,of the performance of new sample preparation Method's. Data are available via PotegmeXchange under identifier PXD005405.
WOS关键词SAMPLE PREPARATION METHOD ; SODIUM DODECYL-SULFATE ; PROTEOMIC ANALYSIS ; MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; PROTEIN ; QUANTIFICATION ; SDS ; ELECTROPHORESIS ; PRECIPITATION
资助项目National Natural Science Foundation of China[21375138] ; National Natural Science Foundation of China[31570830] ; Ministry of Science and Technology of China[2013ZX09507001] ; "one hundred talent program" of the Chinese Academy of Sciences[00000000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12030203] ; Innovation Project of Instrument and Equipment Function Development[YZ201542] ; Innovation Project of Instrument and Equipment Function Development of the Chinese Academy of Sciences from the Bureau of Goods, Chinese Academy of Sciences[00000000] ; E-institute of Shanghai Municipal Education Committee[E03008] ; Open Project Program of Jiangsu Key Laboratory of Pediatric Respiratory Disease, Nanjing University of Chinese Medicine[JKLPRD201404]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000403036800023
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/272618]  
专题分析化学研究室
通讯作者Zhou, Hu
作者单位1.East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Meilong Rd, Shanghai 200237, Peoples R China;
2.Chinese Acad Sci, Dept Analyt Chem, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China;
3.Shanghai Univ, Coll Sci, Dept Chem, Shanghai 200444, Peoples R China;
4.Thermo Fisher Sci China Co Ltd, 6 Bldg,27 Xinjinqiao Rd, Shanghai 201206, Peoples R China;
5.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China;
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
推荐引用方式
GB/T 7714		 Gao, Jing,Zhong, Shaoyun,Zhou, Yanting,et al. Comparative Evaluation of Small Molecular Additives and Their Effects on Peptide/Protein Identification[J]. ANALYTICAL CHEMISTRY,2017,89(11):5785-5793.
APA Gao, Jing.,Zhong, Shaoyun.,Zhou, Yanting.,He, Han.,Peng, Shuying.,...&Zhou, Hu.(2017).Comparative Evaluation of Small Molecular Additives and Their Effects on Peptide/Protein Identification.ANALYTICAL CHEMISTRY,89(11),5785-5793.
MLA Gao, Jing,et al."Comparative Evaluation of Small Molecular Additives and Their Effects on Peptide/Protein Identification".ANALYTICAL CHEMISTRY 89.11(2017):5785-5793.

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来源：上海药物研究所

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