Odorranalectin Is a Small Peptide Lectin with Potential for Drug Delivery and Targeting
文献类型：期刊论文
| 作者 | Li, Jianxu2,5; Wu, Hongbing4; Hong, Jing2,3; Xu, Xueqing2,5; Yang, Hailong5; Wu, Bingxian4; Wang, Yipeng2,5; Zhu, Jianhua4; Lai, Ren5; Jiang, Xinguo4 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2008-06-11 |
| 卷号 | 3期号:6 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0002381 |
| 文献子类 | Article |
| 英文摘要 | Background: Lectins are sugar-binding proteins that specifically recognize sugar complexes. Based on the specificity of protein-sugar interactions, different lectins could be used as carrier molecules to target drugs specifically to different cells which express different glycan arrays. In spite of lectin's interesting biological potential for drug targeting and delivery, a potential disadvantage of natural lectins may be large size molecules that results in immunogenicity and toxicity. Smaller peptides which can mimic the function of lectins are promising candidates for drug targeting. Principal Findings: Small peptide with lectin-like behavior was screened from amphibian skin secretions and its structure and function were studied by NMR, NMR-titration, SPR and mutant analysis. A lectin-like peptide named odorranalectin was identified from skin secretions of Odorrana grahami. It was composed of 17 aa with a sequence of YASPKCFRYPNGVLACT. L-fucose could specifically inhibit the haemagglutination induced by odorranalectin. (125)I-odorranalectin was stable in mice plasma. In experimental mouse models, odorranalectin was proved to mainly conjugate to liver, spleen and lung after i.v. administration. Odorranalectin showed extremely low toxicity and immunogenicity in mice. The small size and single disulfide bridge of odorranalectin make it easy to manipulate for developing as a drug targeting system. The cyclic peptide of odorranalectin disclosed by solution NMR study adopts a beta-turn conformation stabilized by one intramolecular disulfide bond between Cys6-Cys16 and three hydrogen bonds between Phe7-Ala15, Tyr9-Val13, Tyr9-Gly12. Residues K5, C6, F7, C16 and T17 consist of the binding site of L-fucose on odorranalectin determined by NMR titration and mutant analysis. The structure of odorranalectin in bound form is more stable than in free form. Conclusion: These findings identify the smallest lectin so far, and show the application potential of odorranalectin for drug delivery and targeting. It also disclosed a new strategy of amphibian anti-infection. |
| 资助项目 | Ministry of Science and Technology of the People's Republic of China[2007AA02Z192] ; Chinese Academy of Sciences[KSCX2-YW-G-024] ; Chinese Academy of Sciences[KSCX2-YW-R-20] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000263248800021 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272892]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Li, Jianxu |
| 作者单位 | 1.Univ Liverpool, Sch Biol Sci, Liverpool, Merseyside, England; 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China; 4.Fudan Univ, Sch Pharm, Shanghai, Peoples R China; 5.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Biotoxin Units, Kunming, Yunnan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li, Jianxu,Wu, Hongbing,Hong, Jing,et al. Odorranalectin Is a Small Peptide Lectin with Potential for Drug Delivery and Targeting[J]. PLOS ONE,2008,3(6). |
| APA | Li, Jianxu.,Wu, Hongbing.,Hong, Jing.,Xu, Xueqing.,Yang, Hailong.,...&Rees, Huw H..(2008).Odorranalectin Is a Small Peptide Lectin with Potential for Drug Delivery and Targeting.PLOS ONE,3(6). |
| MLA | Li, Jianxu,et al."Odorranalectin Is a Small Peptide Lectin with Potential for Drug Delivery and Targeting".PLOS ONE 3.6(2008). |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。