Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances
文献类型：期刊论文
| 作者 | Liu Xue-Hui; Lin Dong-Hai |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2007-03 |
| 卷号 | 25期号:3页码:411-416 |
| 关键词 | NMR resonance overlapping amide-exchange-rate-edited SEA presat TROSY |
| ISSN号 | 1001-604X |
| DOI | 10.1002/cjoc.200790079 |
| 文献子类 | Article |
| 英文摘要 | This paper describes an amide-exchange-rate-edited (AERE) NMR method that can effectively alleviate the problem of resonance overlap for proteins and peptides. This method exploits the diversity of amide proton exchange rates and consists of two complementary experiments: (1) SEA (solvent exposed amide)-type NMR experiments to map exchangeable surface residues whose amides are not involved in hydrogen bonding, and (2) pre-sat-type NMR experiments to map solvent inaccessibly buried residues or nonexchangeable residues located in hydrogen-bonded secondary structures with properly controlled saturation transfer via amide proton exchanges with the solvent. This method separates overlapping resonances in a spectrum into two complementary spectra. The AERE-NMR method was demonstrated with a sample of N-15/C-13/H-2(70%) labeled ribosome-inactivating protein trichosanthin of 247 residues. |
| WOS关键词 | SOLVENT-EXPOSED AMIDES ; PROTEINS ; GRADIENT ; TROSY ; WATER |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000244805800028 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273312]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Liu Xue-Hui |
| 作者单位 | 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Xue-Hui,Lin Dong-Hai. Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances[J]. CHINESE JOURNAL OF CHEMISTRY,2007,25(3):411-416. |
| APA | Liu Xue-Hui,&Lin Dong-Hai.(2007).Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances.CHINESE JOURNAL OF CHEMISTRY,25(3),411-416. |
| MLA | Liu Xue-Hui,et al."Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances".CHINESE JOURNAL OF CHEMISTRY 25.3(2007):411-416. |
入库方式： OAI收割
来源：上海药物研究所
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