中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances

文献类型:期刊论文

作者Liu Xue-Hui; Lin Dong-Hai
刊名CHINESE JOURNAL OF CHEMISTRY
出版日期2007-03
卷号25期号:3页码:411-416
关键词NMR resonance overlapping amide-exchange-rate-edited SEA presat TROSY
ISSN号1001-604X
DOI10.1002/cjoc.200790079
文献子类Article
英文摘要This paper describes an amide-exchange-rate-edited (AERE) NMR method that can effectively alleviate the problem of resonance overlap for proteins and peptides. This method exploits the diversity of amide proton exchange rates and consists of two complementary experiments: (1) SEA (solvent exposed amide)-type NMR experiments to map exchangeable surface residues whose amides are not involved in hydrogen bonding, and (2) pre-sat-type NMR experiments to map solvent inaccessibly buried residues or nonexchangeable residues located in hydrogen-bonded secondary structures with properly controlled saturation transfer via amide proton exchanges with the solvent. This method separates overlapping resonances in a spectrum into two complementary spectra. The AERE-NMR method was demonstrated with a sample of N-15/C-13/H-2(70%) labeled ribosome-inactivating protein trichosanthin of 247 residues.
WOS关键词SOLVENT-EXPOSED AMIDES ; PROTEINS ; GRADIENT ; TROSY ; WATER
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000244805800028
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/273312]  
专题分析化学研究室
通讯作者Liu Xue-Hui
作者单位1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Liu Xue-Hui,Lin Dong-Hai. Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances[J]. CHINESE JOURNAL OF CHEMISTRY,2007,25(3):411-416.
APA Liu Xue-Hui,&Lin Dong-Hai.(2007).Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances.CHINESE JOURNAL OF CHEMISTRY,25(3),411-416.
MLA Liu Xue-Hui,et al."Amide-exchange-rate-edited NMR (AERE-NMR) experiment: A novel method for resolving overlapping resonances".CHINESE JOURNAL OF CHEMISTRY 25.3(2007):411-416.

入库方式: OAI收割

来源:上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。