Low temperature induced de-differentiation of astrocytes
文献类型：期刊论文
| 作者 | Yu, Tao; Cao, Guan; Feng, Linyin
|
| 刊名 | JOURNAL OF CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 99期号:4页码:1096-1107 |
| 关键词 | radial glial-like cells (RGLCs) de-differentiation neuronal migration radial glia |
| ISSN号 | 0730-2312 |
| DOI | 10.1002/jcb.20979 |
| 文献子类 | Article |
| 英文摘要 | Radial glial cells are astrocyte precursors, which are transiently present in the developing central nervous system and transform eventually into astrocytes in the cerebral cortex and into Bergmann glia in the cerebellum. Previous reports indicate that the transformation from radial glia to astrocytes can be reversed by diffusible chemical signals derived from embryonic forebrain in vitro and by freezing injury in vivo. But there is no direct evidence proving that mature astrocytes can de-differentiate into radial glial cells. Here we show that purified astrocytes could de-differentiate into radial glial-like cells (RGLCs) in vitro with freeze-thaw stimulation. RGLCs had the expression of markers for radial glia including Nestin and Pax6, and astrocyte markers, the glial fibrillary acidic protein and Vimentin. Cortical neurons, when co-cultured with RGLCs, migrated along the processes of RGLCs at an average speed of 26.26 +/- 3.36 mu m/h. Moreover, the proliferation of RGLCs was significantly promoted by epidermal growth factor (EGF) at the concentration of 10-30 ng/ml. These results reveal that low temperature induces astrocytes to de-differentiate into immature RGLCs, which provides an in vitro model to investigate mechanisms of astroglial cells de-differentiation. |
| WOS关键词 | RADIAL GLIAL-CELLS ; NEURAL STEM-CELLS ; CENTRAL-NERVOUS-SYSTEM ; SUBVENTRICULAR ZONE ; CEREBRAL-CORTEX ; SPINAL-CORD ; PROLIFERATION INVITRO ; INTERMEDIATE FILAMENT ; EMBRYONIC FOREBRAIN ; PROGENITOR CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000241689200010 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273458]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Feng, Linyin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Neuropharmacol, Shanghai 200031, Peoples R China 3.Shanghai Univ, Sch Life Sci, Dept Mol Biol, Shanghai 200444, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Tao,Cao, Guan,Feng, Linyin. Low temperature induced de-differentiation of astrocytes[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2006,99(4):1096-1107. |
| APA | Yu, Tao,Cao, Guan,&Feng, Linyin.(2006).Low temperature induced de-differentiation of astrocytes.JOURNAL OF CELLULAR BIOCHEMISTRY,99(4),1096-1107. |
| MLA | Yu, Tao,et al."Low temperature induced de-differentiation of astrocytes".JOURNAL OF CELLULAR BIOCHEMISTRY 99.4(2006):1096-1107. |
入库方式： OAI收割
来源：上海药物研究所
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