Activation of adenosine A(1) receptor modulates dopamine D-1 receptor activity in stably cotransfected human embryonic kidney 293 cells
文献类型：期刊论文
| 作者 | Cao, Yan; Sun, Wan-Chun; Jin, Lei; Xie, Ke-Qiang; Zhu, Xing-Zu |
| 刊名 | EUROPEAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 2006-10-24 |
| 卷号 | 548期号:1-3页码:29-35 |
| 关键词 | dopamine D-1 receptor adenosine A(1) receptor modulation CRE-SEAP reporter gene |
| ISSN号 | 0014-2999 |
| DOI | 10.1016/j.ejphar.2006.07.051 |
| 文献子类 | Article |
| 英文摘要 | The antagonistic interactions between adenosine A(1) receptors and dopamine D-1 receptors were studied in a human embryonic kidney 293 cell line stably cotransfected with human adenosine A(1) receptor and dopamine D-1 receptor cDNAs. In the cotransfected cells, but not in control cells only transfected with dopamine D-1 receptors, adenosine A(1) receptor agonist N-6-cyclopentyladenosine (CPA, 10 mu M) increased the K-d of dopamine D-1 receptor antagonist [N-methyl-H-3]R(+)-7-Chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine ([H-3] SCH23390) without affecting the B-max. Moreover, CPA induced a concentration-dependent decrease in the affinity of dopamine D-1 receptors for the agonist (+/-)-1-Phenyl-2,3,4,5-tetrahydro-(1H)-3-benzazepine-7,8-diol hydrochloride (SKF38393) and inhibited dopamine D-1 receptor-mediated cyclic AMP response element recruitment. Furthermore, pertussis toxin treatment completely counteracted the effects of low concentrations of CPA but only partially counteracted the effects of high concentrations of CPA. These results suggest that adenosine A(1) receptors antagonistically modulate dopamine D-1 receptors at the level of receptor binding and the second messenger generation. Furthermore, the antagonistic interactions between these two receptors induced by low concentrations of CPA might have a different manner with those induced by high concentrations of CPA. (c) 2006 Elaevier B.V. All rights reserved. |
| WOS关键词 | PROTEIN-COUPLED RECEPTORS ; TERNARY COMPLEX MODEL ; INTEGRATIVE MECHANISM ; ADENYLATE-CYCLASE ; LINKED RECEPTORS ; BINDING ; A(2A) ; SYSTEM ; ASSAY ; D1 |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000241172600004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273467]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Zhu, Xing-Zu |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Yan,Sun, Wan-Chun,Jin, Lei,et al. Activation of adenosine A(1) receptor modulates dopamine D-1 receptor activity in stably cotransfected human embryonic kidney 293 cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2006,548(1-3):29-35. |
| APA | Cao, Yan,Sun, Wan-Chun,Jin, Lei,Xie, Ke-Qiang,&Zhu, Xing-Zu.(2006).Activation of adenosine A(1) receptor modulates dopamine D-1 receptor activity in stably cotransfected human embryonic kidney 293 cells.EUROPEAN JOURNAL OF PHARMACOLOGY,548(1-3),29-35. |
| MLA | Cao, Yan,et al."Activation of adenosine A(1) receptor modulates dopamine D-1 receptor activity in stably cotransfected human embryonic kidney 293 cells".EUROPEAN JOURNAL OF PHARMACOLOGY 548.1-3(2006):29-35. |
入库方式： OAI收割
来源：上海药物研究所
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