Inhibitory effects of procyanidin B-2 dimer on lipid-laden macrophage formation
文献类型：期刊论文
| 作者 | Chen, Dong-Mei; Cai, Xin; Kwik-Uribe, Catherine L.; Zeng, Rong; Zhu, Xing-Zu |
| 刊名 | JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
 |
| 出版日期 | 2006-08 |
| 卷号 | 48期号:2页码:54-70 |
| 关键词 | lipid-laden macrophages oxidized low density lipoprotein lectin-like oxidized low-density lipoprotein receptor-1 proteomics atherosclerosis Kyoto Encyclopedia of Genes and Genomes |
| ISSN号 | 0160-2446 |
| DOI | 10.1097/01.fjc.0000242052.60502.21 |
| 文献子类 | Article |
| 英文摘要 | A proteomic analysis of procyanidin B-2 isolated from cocoa against oxidized low-density lipoprotein-induced lipid-laden macrophage formation was performed. Of approximate to 400 detected proteins, 12 were differentially expressed as a result of B-2 treatment. They were subsequently identified by liquid chromatography-electrospray ionization-tandem mass spectrometry and the SWISS-PROT database. Further reverse transcriptase-polymerase chain reaction and Western blot analysis revealed that B-2 strongly inhibited arachidonic acid inflammatory reactions, apoptosis, and their coupled mitogen-activated protein kinase and NF-kappa B pathways. To highlight proteins or genes with similar expressed patterns and similarly biological function induced by B-2 in lipid-laden macrophages, a cluster and Kyoto Encyclopedia of Genes and Genomes pathway analysis were performed. The data were mapped to multiple pathways. Further validation of the bioinformatic results revealed that activation of Win signaling may contribute to the cardioprotection of B-2. The differentially expressed genes and proteins mentioned above induced by B-2 are through regulating nuclear transcription factors, activating peroxisome proliferator-activated receptor-gamma and inhibiting AP-1 mRNA expressions. These in vitro data help to interpret the beneficial effects of B-2 in reducing the risk of atherosclerosis after consumption of flavonoid-rich foods. Many differentially expressed genes induced by B-2 help to uncover novel targets and may help to target disease interactions in atherosclerosis in the future. |
| WOS关键词 | LOW-DENSITY-LIPOPROTEIN ; SMOOTH-MUSCLE-CELLS ; NF-KAPPA-B ; FOAM CELL ; IN-VITRO ; MASS-SPECTROMETRY ; ATHEROSCLEROSIS ; PROTEIN ; EXPRESSION ; EXTRACT |
| WOS研究方向 | Cardiovascular System & Cardiology ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000240813300010 |
| 出版者 | LIPPINCOTT WILLIAMS & WILKINS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273529]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Zhu, Xing-Zu |
| 作者单位 | 1.Mars Inc, Hackensack, NJ USA 2.Res Ctr Proteome Anal, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Dept Pharmacol, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Dong-Mei,Cai, Xin,Kwik-Uribe, Catherine L.,et al. Inhibitory effects of procyanidin B-2 dimer on lipid-laden macrophage formation[J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY,2006,48(2):54-70. |
| APA | Chen, Dong-Mei,Cai, Xin,Kwik-Uribe, Catherine L.,Zeng, Rong,&Zhu, Xing-Zu.(2006).Inhibitory effects of procyanidin B-2 dimer on lipid-laden macrophage formation.JOURNAL OF CARDIOVASCULAR PHARMACOLOGY,48(2),54-70. |
| MLA | Chen, Dong-Mei,et al."Inhibitory effects of procyanidin B-2 dimer on lipid-laden macrophage formation".JOURNAL OF CARDIOVASCULAR PHARMACOLOGY 48.2(2006):54-70. |
入库方式： OAI收割
来源：上海药物研究所
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