Intracellular dopamine oxidation mediates rotenone-induced apoptosis in PC12 cells
文献类型：期刊论文
| 作者 | Liu, HQ; Zhu, XZ; Weng, EQ |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2005-01 |
| 卷号 | 26期号:1页码:17-26 |
| 关键词 | Parkinson disease rotenone reserpine deprenyl reactive oxygen species neurotoxicity |
| ISSN号 | 1671-4083 |
| DOI | 10.1117/j.1745-7254.2005.00003.x |
| 文献子类 | Article |
| 英文摘要 | Aim: To study the role of dopamine (DA) in rotenone-induced neurotoxicity in PC 12 cells. Methods: Cell viability was assessed by detecting the leakage of lactate dehydrogenase (LDH) into the medium. Apoptosis rate was measured by flow cytometry. Caspase-3-like activity was measured by fluorescence assay using the probe Ac-DEVD-AMC. The level of intracellular hydrogen peroxide and other peroxides in PC12 cells were quantified by loading cells with 2'-7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) in fluorescence assay. Lactic acid was measured spectrophotometrically. The DA levels in PC12 cells were determined by HPLC-ECD. Results: A 48-h incubation of PC12 cells with rotenone caused an apoptotic cell death and elevated intracellular reactive oxygen species (ROS) and lactic acid accumulation. Intracellular DA depletion with reserpine significantly attenuated rotenone-induced ROS accumulation and apoptotic cell death. No change was found in rotenone-induced ROS accumulation when cells were co-treated with deprenyl. Brief treatment with reserpine at the end of rotenone treatment had no effect on rotenone-induced neurotoxicity. However, when cells were first incubated with deprenyl, a monoamine oxidase-B inhibitor for 30 min then co-incubated with rotenone plus deprenyl, a brief treatment with reserpine enhanced cell injury. Conclusion: Rotenone-induced apoptosis in PC12 cells was mediated by intracellular dopamine oxidation. |
| WOS关键词 | PARKINSONS-DISEASE ; ALPHA-SYNUCLEIN ; NITRIC-OXIDE ; HYDROGEN-PEROXIDE ; SUBSTANTIA-NIGRA ; NERVE-TERMINALS ; MITOCHONDRIA ; TOXICITY ; BRAIN ; NEUROTOXICITY |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000226426600003 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273945]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | 1.E China Normal Univ, Dept Environm Sci & Technol, Shanghai 200062, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Dept Pharmacol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, HQ,Zhu, XZ,Weng, EQ. Intracellular dopamine oxidation mediates rotenone-induced apoptosis in PC12 cells[J]. ACTA PHARMACOLOGICA SINICA,2005,26(1):17-26. |
| APA | Liu, HQ,Zhu, XZ,&Weng, EQ.(2005).Intracellular dopamine oxidation mediates rotenone-induced apoptosis in PC12 cells.ACTA PHARMACOLOGICA SINICA,26(1),17-26. |
| MLA | Liu, HQ,et al."Intracellular dopamine oxidation mediates rotenone-induced apoptosis in PC12 cells".ACTA PHARMACOLOGICA SINICA 26.1(2005):17-26. |
入库方式： OAI收割
来源：上海药物研究所
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