Clean SEA-TROSY experiments to map solvent exposed amides in large proteins
文献类型：期刊论文
| 作者 | Lin, DH |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2004-12 |
| 卷号 | 22期号:12页码:1395-1398 |
| 关键词 | proteins solvent accessibility hydrogen exchange TROSY NMR |
| ISSN号 | 1001-604X |
| 文献子类 | Article |
| 英文摘要 | It is well known that the SEA-TROSY experiment could alleviate some of the problems of resonance overlap in N-15/H-2 labeled proteins as it was designed to selectively map solvent exposed amide protons. However, SEA-TROSY spectra may be contaminated with exchange-relayed NOE contributions from fast exchanged hydroxyl or amine protons and contributions from longitudinal relaxation. Also, perdeuteration of the protein sample is a prerequisite for this experiment. In this communication, a modified version, clean SEA-TROSY, was proposed to eliminate these artifacts and to allow the experiment to be applied to protonated or partially deuterated proteins and protein complexes. |
| WOS关键词 | PROTON-EXCHANGE RATES ; HYDROGEN-EXCHANGE ; WATER ; SPECTROSCOPY ; NMR ; GRADIENT ; COMPLEX ; HSQC ; NOES ; PM |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000225724600002 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273986]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Lin, DH |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Meteria Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, DH. Clean SEA-TROSY experiments to map solvent exposed amides in large proteins[J]. CHINESE JOURNAL OF CHEMISTRY,2004,22(12):1395-1398. |
| APA | Lin, DH.(2004).Clean SEA-TROSY experiments to map solvent exposed amides in large proteins.CHINESE JOURNAL OF CHEMISTRY,22(12),1395-1398. |
| MLA | Lin, DH."Clean SEA-TROSY experiments to map solvent exposed amides in large proteins".CHINESE JOURNAL OF CHEMISTRY 22.12(2004):1395-1398. |
入库方式： OAI收割
来源：上海药物研究所
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