中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Clean SEA-TROSY experiments to map solvent exposed amides in large proteins

文献类型:期刊论文

作者Lin, DH
刊名CHINESE JOURNAL OF CHEMISTRY
出版日期2004-12
卷号22期号:12页码:1395-1398
关键词proteins solvent accessibility hydrogen exchange TROSY NMR
ISSN号1001-604X
文献子类Article
英文摘要It is well known that the SEA-TROSY experiment could alleviate some of the problems of resonance overlap in N-15/H-2 labeled proteins as it was designed to selectively map solvent exposed amide protons. However, SEA-TROSY spectra may be contaminated with exchange-relayed NOE contributions from fast exchanged hydroxyl or amine protons and contributions from longitudinal relaxation. Also, perdeuteration of the protein sample is a prerequisite for this experiment. In this communication, a modified version, clean SEA-TROSY, was proposed to eliminate these artifacts and to allow the experiment to be applied to protonated or partially deuterated proteins and protein complexes.
WOS关键词PROTON-EXCHANGE RATES ; HYDROGEN-EXCHANGE ; WATER ; SPECTROSCOPY ; NMR ; GRADIENT ; COMPLEX ; HSQC ; NOES ; PM
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000225724600002
出版者SCIENCE CHINA PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/273986]  
专题分析化学研究室
通讯作者Lin, DH
作者单位Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Meteria Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Lin, DH. Clean SEA-TROSY experiments to map solvent exposed amides in large proteins[J]. CHINESE JOURNAL OF CHEMISTRY,2004,22(12):1395-1398.
APA Lin, DH.(2004).Clean SEA-TROSY experiments to map solvent exposed amides in large proteins.CHINESE JOURNAL OF CHEMISTRY,22(12),1395-1398.
MLA Lin, DH."Clean SEA-TROSY experiments to map solvent exposed amides in large proteins".CHINESE JOURNAL OF CHEMISTRY 22.12(2004):1395-1398.

入库方式: OAI收割

来源:上海药物研究所

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