Erk1/2 but not PI3K pathway is required for neurotrophin 3-induced oligodendrocyte differentiation of post-natal neural stem cells
文献类型：期刊论文
| 作者 | Hu, XH; Jin, L; Feng, LY
|
| 刊名 | JOURNAL OF NEUROCHEMISTRY
 |
| 出版日期 | 2004-09 |
| 卷号 | 90期号:6页码:1339-1347 |
| 关键词 | differentiation extracellular signal-related kinase1/ 2 neural stem cell neurotrophin 3 oligodendrocyte lineage gene-1 post-natal |
| ISSN号 | 0022-3042 |
| DOI | 10.1111/j.1471-4159.2004.02594.x |
| 文献子类 | Article |
| 英文摘要 | Neurotrophin 3 (NT3) induces mouse cortical stem cells to an asymmetric division from a symmetric division, suggesting that NT3 may work as an early differentiative signal for neural stem cells (NSCs). Here, using cultured post-natal hippocampal stem cells as a model, we demonstrated that NT3-stimulation causes NSCs to differentiate into oligodendrocyte precursors (OLPs) through an extracellular signal-related kinase1/2 (Erk1/2)-dependent pathway. Following the treatment of NT3 for 24 h, NSCs differentiated into more OLPs and fewer neurons, whereas the proliferation and survival of OLPs were not affected. NT3 induced a series of intracellular responses including enhancement of phosphorylation of Erk1/2 or Akt and increase of expression of oligodendrocyte lineage gene (Olig)-1, a transcriptional factor known to participate in oligodendrocyte development. Application of U0126, a specific inhibitor of MEK1/2 which are upstream to Erk1/2, blocked the phosphorylation of Erk1/2, suppressed the expression of Olig-1 and prevented NSC differentiation into OLPs in response to NT3 stimulation. Blockade of TrkC also inhibited the differentiation of NSCs to OLPs induced by NT3. However, administration of LY294002, an inhibitor of phosphatidylinositol 3 kinase (PI3K), blocked the phosphorylation of Akt but did not affect the effect of NT3 on the expression of Olig-1 and on NSC differentiation into OLPs. Taken together, these results suggest that NT3 induce NSCs to differentiate into OLPs by enhancing the expression of Olig-1 through an Erk1/2-dependent pathway. |
| WOS关键词 | BHLH TRANSCRIPTION FACTORS ; SIGNALING PATHWAYS ; NEURONAL DIFFERENTIATION ; INDEPENDENT MECHANISMS ; NERVOUS-SYSTEM ; GROWTH-FACTOR ; SURVIVAL ; ACTIVATION ; LINEAGE ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000223642600007 |
| 出版者 | BLACKWELL PUBLISHING LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274028]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Feng, LY |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Grad Sch,Inst Neurosci, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, XH,Jin, L,Feng, LY. Erk1/2 but not PI3K pathway is required for neurotrophin 3-induced oligodendrocyte differentiation of post-natal neural stem cells[J]. JOURNAL OF NEUROCHEMISTRY,2004,90(6):1339-1347. |
| APA | Hu, XH,Jin, L,&Feng, LY.(2004).Erk1/2 but not PI3K pathway is required for neurotrophin 3-induced oligodendrocyte differentiation of post-natal neural stem cells.JOURNAL OF NEUROCHEMISTRY,90(6),1339-1347. |
| MLA | Hu, XH,et al."Erk1/2 but not PI3K pathway is required for neurotrophin 3-induced oligodendrocyte differentiation of post-natal neural stem cells".JOURNAL OF NEUROCHEMISTRY 90.6(2004):1339-1347. |
入库方式： OAI收割
来源：上海药物研究所
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