中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Expression and enzyme activity determination of human cyclooxygenase-1 and-2 in a baculovirus-insect cell system

文献类型:期刊论文

作者Zhang, WY; Yang, XN; Jin, DZ; Zhu, XZ
刊名ACTA PHARMACOLOGICA SINICA
出版日期2004-08
卷号25期号:8页码:1000-1006
关键词baculoviruses prostaglandin-endoperoxide synthase non-steroidal anti inflammatory agents prostaglandins drug inhibition
ISSN号1671-4083
文献子类Article
英文摘要AIM: To develop an in vitro intact cell-based assay for screening selective cyclooxygenase inhibitors. METHODS: Human cyclooxygenase-1 (hCOX-1) and cyclooxygenase-2 (hCOX-2) genes were cloned from human monocyte cell line THP-1 cells and expressed in Spodoptera frugiperda (sf9) insect cell line by Bac-to-Bac baculovirus expression systems. Infected sf9 cells were harvested 24 h post-infection (hpi), and distributed to a 24-well plate, preincubated with various nonsteroidal anti-inflammatory drugs, and challenged with 10 mmol/L arachidonic acid; the cyclooxygenase activity was assessed indirectly by prostaglandin E(2)-specific radioimmunoassay. RESULTS: Polymerase chain reaction detection demonstrated that hCOX-1 and hCOX-2 were transposed to the bacmid. Western blot analysis showed that infected sf9 cells could express hCOX-1 and hCOX-2 proteins. Radioimmunoassay demonstrated that both recombinant proteins functioned well in sf9 cells. CONCLUSION: Human cyclooxygenase-1 and cyclooxygenase-2 were successfully expressed in sf9 insect cell line. It can be utilized for the identification of potent and selective inhibitors of hCOX-1 and/or hCOX-2.
WOS关键词SELECTIVE-INHIBITION ; ANTIINFLAMMATORY DRUGS ; SYNTHASE-1 ; BIOSYNTHESIS ; COX-2
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
CSCD记录号CSCD:1845122
WOS记录号WOS:000223240900005
出版者SHANGHAI INST MATERIA MEDICA
源URL[http://119.78.100.183/handle/2S10ELR8/274038]  
专题药理学第二研究室
通讯作者Zhu, XZ
作者单位Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Zhang, WY,Yang, XN,Jin, DZ,et al. Expression and enzyme activity determination of human cyclooxygenase-1 and-2 in a baculovirus-insect cell system[J]. ACTA PHARMACOLOGICA SINICA,2004,25(8):1000-1006.
APA Zhang, WY,Yang, XN,Jin, DZ,&Zhu, XZ.(2004).Expression and enzyme activity determination of human cyclooxygenase-1 and-2 in a baculovirus-insect cell system.ACTA PHARMACOLOGICA SINICA,25(8),1000-1006.
MLA Zhang, WY,et al."Expression and enzyme activity determination of human cyclooxygenase-1 and-2 in a baculovirus-insect cell system".ACTA PHARMACOLOGICA SINICA 25.8(2004):1000-1006.

入库方式: OAI收割

来源:上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。