Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum
文献类型：期刊论文
| 作者 | Bao, XF; Zhen, Y; Ruan, L; Fang, JN |
| 刊名 | CHEMICAL & PHARMACEUTICAL BULLETIN
 |
| 出版日期 | 2002-05 |
| 卷号 | 50期号:5页码:623-629 |
| 关键词 | Ganoderma lucidum polysaccharide T lymphocyte-stimulating activity sulfation carboxymethylation solution conformation |
| ISSN号 | 0009-2363 |
| DOI | 10.1248/cpb.50.623 |
| 文献子类 | Article |
| 英文摘要 | The hot-water extract of the spores of Ganoderma lucidum was shown to have a stimulating effect on concanavalin A-induced mitogenic activity of T lymphocytes. Bioassay-guided separation led to the isolation of a polysaccharide with potent T lymphocyte-stimulating activity by ethanol fractionation, anion-exchange, and size-exclusion chromatography. Based on the composition and methylation analyses, periodate oxidation, Smith degradation, and NMR spectroscopy, the native polysaccharide was shown to be a beta-D-(1-->3)-glucan with branches of terminal glucosyl residues substituted at C-6 of the glucose residues in the main chain. The branching ratio is approximately 20%. A series of sulfated or carboxymethylated derivatives were prepared and their structural features were elucidated by chemical and spectral analyses. The solution conformation and T lymphocyte proliferation effect of the glucans before and after derivatization were compared and discussed. The data obtained indicate that the introduction of ionic groups would significantly affect the original conformation of the native glucan in aqueous solution and further affect T lymphocyte-stimulating activity. The triple-helical structure of the glucans, the nature of the ionic groups, and the density of negative charge were considered to be closely related to this activity. |
| WOS关键词 | ANTITUMOR-ACTIVITY ; INHIBITORY ACTIVITY ; GLUCAN ; CURDLAN ; (1->3)-BETA-D-GLUCANS ; DERIVATIVES ; TRITERPENES ; BEHAVIOR ; SULFATE ; HIV-1 |
| WOS研究方向 | Pharmacology & Pharmacy ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000175370700011 |
| 出版者 | PHARMACEUTICAL SOC JAPAN |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274365]  |
| 专题 | 药理学第三研究室 天然药物化学研究室 |
| 通讯作者 | Fang, JN |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, XF,Zhen, Y,Ruan, L,et al. Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum[J]. CHEMICAL & PHARMACEUTICAL BULLETIN,2002,50(5):623-629. |
| APA | Bao, XF,Zhen, Y,Ruan, L,&Fang, JN.(2002).Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum.CHEMICAL & PHARMACEUTICAL BULLETIN,50(5),623-629. |
| MLA | Bao, XF,et al."Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum".CHEMICAL & PHARMACEUTICAL BULLETIN 50.5(2002):623-629. |
入库方式： OAI收割
来源：上海药物研究所
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