Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis
文献类型：期刊论文
| 作者 | Bao, XF; Wang, Z ; Fang, JN; Li, XY
|
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2002-03 |
| 卷号 | 68期号:3页码:237-243 |
| 关键词 | Cuscuta chinensis Convolvulaceae acidic polysaccharide lymphocyte proliferation antibody production free radical PC12 cell |
| ISSN号 | 0032-0943 |
| DOI | 10.1055/s-2002-23133 |
| 文献子类 | Article |
| 英文摘要 | Three crude polysaccharide fractions, named CS-A, CS-B and CS-C, were prepared from the seeds of Cuscuta chinensis by hot water extraction and diluted alkali extraction subsequently, then EtOH precipitation, and tested for lymphocyte proliferation activity. CS-A showed a stimulating effect on concanavalin A or lipopolysaccharide induced mitogenic activity of lymphocytes. An acidic polysaccharide (CS-A-3beta) was purified from CS-A by anion exchange and gel filtration chromatography. The polysaccharide showed potent stimulating effects on lymphocyte proliferation and antibody production, but did not significantly influence the serum IgG level. Its structural features were elucidated by methylation analysis, partial acid hydrolysis, 1 D and 2D NMR and ESI-mass spectroscopy. The data obtained indicated that CS-A-3beta had a backbone consisting of alpha-D-1,4-linked GalpA residues and beta-L-1,2-linked Rhap residues with branches at C-4 of Rhap residues and C-3 of GalpA residues, composed of arabinogalactan and glucobiose. This structure is typical for a pectic polysaccharide of the rhamnogalacturonan type. In addition, the effect of CS-A, CS-B, CS-C and CS-A-3beta on hydrogen peroxide induced cell lesion in rat pheochromocytoma line PC 12 was investigated. The results indicated that, besides its immunostimulating activity, CS-A-3beta had a protective effect against free radical-induced cell toxicity. |
| WOS关键词 | ROOTS ; GLC |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000174883700010 |
| 出版者 | GEORG THIEME VERLAG KG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274384]  |
| 专题 | 天然药物化学研究室 药理学第三研究室 |
| 通讯作者 | Fang, JN |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, XF,Wang, Z,Fang, JN,et al. Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis[J]. PLANTA MEDICA,2002,68(3):237-243. |
| APA | Bao, XF,Wang, Z,Fang, JN,&Li, XY.(2002).Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis.PLANTA MEDICA,68(3),237-243. |
| MLA | Bao, XF,et al."Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis".PLANTA MEDICA 68.3(2002):237-243. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。