Amyloid-beta 25-35 peptide induces expression of monoamine oxidase B in cultured rat astrocytes
文献类型：期刊论文
| 作者 | Song, W; Zhou, LJ; Zheng, SX; Zhu, XZ |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2000-06 |
| 卷号 | 21期号:6页码:557-563 |
| 关键词 | Alzheimer disease astrocytes amyloid beta-protein monoamine oxidase monoamine oxidase inhibitors oxidative stress |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To investigate the effects of amyloid-beta 25 - 35 peptide (A beta 25 - 35) on monoamine oxidase (MAO) expression and activity in primary cultured rat astrocytes. METHODS: Immunocytochemistry was used to observe the morphological changes in astrocytes. Fluorescence spectrophotometry was used to measure the activity of MAO in astrocytes. The expression of MAO in astrocytes was assayed by RT-PCR. RESULTS: A beta 25 - 35 induced a reactive morphological change in cultured rat astrocytes which was accompanied by increased immunoreactivities for glial fibrillary acidic protein. Treatment with A beta 25 - 35 resulted in an elevation of MAO activity in a dose- and time-dependent manner. A beta 25 - 35-induced enhancement of MAO activity was of type B (MAO-B). The increase in MAO-B activity appeared to be due to an increase in the number of enzyme molecules since kinetic analysis demonstrated a 1.5 fold increase in V-max with no change in K-m. Treatment with A beta 25 - 35 also led to a substantial increase in MAO-B mRNA level in the astrocytes. CONCLUSION: A beta 25 - 35 is able to selectively induce MAO-B expression in rat astrocytes and that the upregulation of MAO-B in A beta 25 - 35-stimulated astrocytes may play an important role in the pathogenesis of Alzheimer's disease. |
| WOS关键词 | QUANTITATIVE ENZYME AUTORADIOGRAPHY ; ALZHEIMERS-DISEASE ; REACTIVE GLIOSIS ; SENILE PLAQUES ; HUMAN-BRAIN ; MECHANISMS ; INVITRO ; PROTEIN |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000087561300011 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274614]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol 1, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, W,Zhou, LJ,Zheng, SX,et al. Amyloid-beta 25-35 peptide induces expression of monoamine oxidase B in cultured rat astrocytes[J]. ACTA PHARMACOLOGICA SINICA,2000,21(6):557-563. |
| APA | Song, W,Zhou, LJ,Zheng, SX,&Zhu, XZ.(2000).Amyloid-beta 25-35 peptide induces expression of monoamine oxidase B in cultured rat astrocytes.ACTA PHARMACOLOGICA SINICA,21(6),557-563. |
| MLA | Song, W,et al."Amyloid-beta 25-35 peptide induces expression of monoamine oxidase B in cultured rat astrocytes".ACTA PHARMACOLOGICA SINICA 21.6(2000):557-563. |
入库方式： OAI收割
来源：上海药物研究所
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