Protective effect of bilobalide against nitric oxide-induced neurotoxicity in PC12 cells
文献类型：期刊论文
| 作者 | Song, W; Guan, HJ; Zhu, XZ; Chen, ZL; Yin, ML; Cheng, XF |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2000-05 |
| 卷号 | 21期号:5页码:415-420 |
| 关键词 | PC12 cells SIN-1 neurotoxins neurons cell survival lipid peroxidation neuroprotective agents antioxidants bilobalide |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To examine the effects of bilobalide on nitric oxide-induced neurotoxicity in pheochromocytoma-derived PC12 cells (PC12 cells). METHODS: PC12 cell survival was monitored by LDH release and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Superoxide dismutases (SOD) and catalase (CAT) activities were measured based on their abilities to inhibit the oxidation of epinephrine by the xanthine-xanthine oxidase system or to decompose H2O2 respectively. The content of malondialdehyde (MDA) was measured by a fluorometric assay to indicate the lipid peroxidation. RESULTS: 3-Morpholinosydnonimine (SIN-1, 50 - 300 mu mol.L-1) induced PC12 cell damage. After the cells had been pretreated with 10 mu mol.L-1 bilobalide for 24 h, the cell viability was increased to 91 % +/- 30 % from 52 % +/- 14 % in SIN-I alone group. Moreover, the activities of SOD and CAT were increased after cells were treated with bilobalide. CONCLUSION: The NO-induced neurotoxicity can be protected by bilobalide in PC12 cells. The bilobalide-induced increase in SOD and CAT activities may serve as one of the mechanisms underlying the neuroprotective effect of bilobalide. |
| WOS关键词 | GINKGO-BILOBA ; SUPEROXIDE-DISMUTASE ; NEURONAL APOPTOSIS ; EXTRACT ; PEROXYNITRITE ; CONSTITUENTS ; DEMENTIA |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000086866500006 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274621]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, W,Guan, HJ,Zhu, XZ,et al. Protective effect of bilobalide against nitric oxide-induced neurotoxicity in PC12 cells[J]. ACTA PHARMACOLOGICA SINICA,2000,21(5):415-420. |
| APA | Song, W,Guan, HJ,Zhu, XZ,Chen, ZL,Yin, ML,&Cheng, XF.(2000).Protective effect of bilobalide against nitric oxide-induced neurotoxicity in PC12 cells.ACTA PHARMACOLOGICA SINICA,21(5),415-420. |
| MLA | Song, W,et al."Protective effect of bilobalide against nitric oxide-induced neurotoxicity in PC12 cells".ACTA PHARMACOLOGICA SINICA 21.5(2000):415-420. |
入库方式： OAI收割
来源：上海药物研究所
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