Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes
文献类型：期刊论文
| 作者 | Liu, X ; Zheng, SX; Zhou, LJ; Zhu, XZ
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2000-01 |
| 卷号 | 21期号:1页码:19-22 |
| 关键词 | basic fibroblast growth factor astrocytes endothelial growth factors cultured cells genetic transcription polymerase chain reaction message RNA immunohistochemistry |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To examine the effect of recombinant human basic fibroblast growth factor(bFGF) on the expression of vascular endothelial growth factor (VEGF) in primary cultured rat astrocytes. METHODS: Semiquantification PCR (SQ-PCR) and immunocytochemistry were used to investigate the effect of bFGF on VEGF mRNA level and protein level, respectively. RESULTS: Treatment with bFGF dose-dependently increased the VEGF mRNA level in astrocytes. The up-regulation of VEGF mRNA induced by bFGF (10 mu g/L) was detected as short as 3-h treatment. The increase of VEGF mRNA level reached the maximum after 24-h treatment with bFGF. The immunocytochemical staining showed that the VEGF protein level in astrocytes also increased after the cells were incubated with bFGF. CONCLUSION: bFGF induced a marked time- and concentration-dependent increase in VEGF expression in primary cultured astrocytes, suggesting that the effect of bFGF on angiogenesis in brain may act partly by up-regulating VEGF expression in astrocytes. |
| WOS关键词 | FOCAL CEREBRAL-ISCHEMIA ; FACTOR FAMILY ; BRAIN ; ANGIOGENESIS ; REDUCTION ; INDUCTION ; FOREBRAIN ; DAMAGE |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000084710700002 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274652]  |
| 专题 | 分析化学研究室 药理学第二研究室 药物安全性评价中心 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol 1, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, X,Zheng, SX,Zhou, LJ,et al. Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes[J]. ACTA PHARMACOLOGICA SINICA,2000,21(1):19-22. |
| APA | Liu, X,Zheng, SX,Zhou, LJ,&Zhu, XZ.(2000).Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes.ACTA PHARMACOLOGICA SINICA,21(1),19-22. |
| MLA | Liu, X,et al."Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes".ACTA PHARMACOLOGICA SINICA 21.1(2000):19-22. |
入库方式： OAI收割
来源：上海药物研究所
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