Up-regulation of LPS-induced iNOS activity in dibutyryl cyclic AMP differentiated rat astrocytes
文献类型：期刊论文
| 作者 | Song, W; Zhu, XZ |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1998-09 |
| 卷号 | 19期号:5页码:462-+ |
| 关键词 | lipopolysaccharides nitric-oxide synthase cyclic AMP cell differentiation up-regulation (Physiology) astrocytes immunohistochemistry |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the effect of dBcAMP on bacterial endotoxin ISS-induced NOS activity. METHODS: Microscopic changes were observed. Nitrite levels were measured by fluorometric assay. NOS activity was measured by citrulline assay. RESULTS: Within 3-4 h after the addition of dBcAMP 1 mmol . L-1 to culture medium, a morphological transformation reminiscent of in vivo, differentiation occurred. Coincubation with LPS and dBcAMP 1 mmol . L-1 resulted in a marked increase in the nitrite production as compared with LPS alone. This increase was concentration- and time-dependent with a maximal effect after 24 h treatment. Nitrite production stimulated by LPS is parallel to the degree of cell differentiation. After a 24-h costimulation with LPS and dBcAMP, L-citrulline formation assay revealed a 3-fold increase in NOS activity over LPS treatment alone, Simultaneous incubation with L-NAME, completely inhibited the stimulation effect of LSS/dBcAMP on nitrite production, Cycloheximide and dactinomycin also suppressed enhancement of NOS activity stimulated by LPS/ dBcAMP, both in nitrite production and citrulline assay, indicating that the enhancement of NOS activity was due to the expression of inducible NOS (iNOS) gene and protein. CONCLUSION: Inflammatory signals can trigger astrocytes to express substantially different levels of iNOS depending on their degree of differentiation. |
| WOS关键词 | NITRIC-OXIDE SYNTHASE ; MOUSE ASTROCYTES ; PRIMARY CULTURES ; CELLS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000075784800017 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274809]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, W,Zhu, XZ. Up-regulation of LPS-induced iNOS activity in dibutyryl cyclic AMP differentiated rat astrocytes[J]. ACTA PHARMACOLOGICA SINICA,1998,19(5):462-+. |
| APA | Song, W,&Zhu, XZ.(1998).Up-regulation of LPS-induced iNOS activity in dibutyryl cyclic AMP differentiated rat astrocytes.ACTA PHARMACOLOGICA SINICA,19(5),462-+. |
| MLA | Song, W,et al."Up-regulation of LPS-induced iNOS activity in dibutyryl cyclic AMP differentiated rat astrocytes".ACTA PHARMACOLOGICA SINICA 19.5(1998):462-+. |
入库方式： OAI收割
来源：上海药物研究所
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