Production of anti-peptide antibody of rat brain nitric-oxide synthase
文献类型：期刊论文
| 作者 | Zhai, R; Zhu, XZ |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1997-05 |
| 卷号 | 18期号:3页码:204-+ |
| 关键词 | nitric-oxide synthase NADPH dehydrogenase peptides epitopes antibodies enzyme-linked immunosorbent assay Western blotting immunohistochemistry cerebellum |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To raise antibody of rat brain nitric-oxide synthase (bNOS) through immunizing animal with a peptide of bNOS that can represent the holoprotein. METHODS: The amino acid sequence for the bNOS was analyzed by GenePro computer program. According to the hydrophilicity, hydrophobicity, antigenicity, and the potentiality to form. protein second structures of alpha-helix, beta-sheet and beta-turn, the structure of bNOS was predicted. The peptide 277 - 287 was selected that was predicted to be in the antigen epitope of bNOS. The peptide was chemically synthesized, coupled to keyhole limphet hemocyanin carrier protein and injected into rabbits to raise antibody. The specificity of the antibody was tested by enzyme- linked immunosorbent assay, immunohistochemistry, and Western blotting. RESULTS: The antibody bound the protein in rat cerebellum extract. In Western blotting, the antibody bound the protein band of 150 kDa in SDS-PAGE, and the binding was inhibited by peptide conjugated with carrier protein, In immunohistochemistry, the stain was collocated with the stain in NADPH-dehydrogenase histochemistry. CONCLUSION: The antibody against the peptide recognized the natural bNOS in rat brain, and the peptide 277 - 287 was located on the surface of bNOS. |
| WOS关键词 | PROTEINS ; PHOSPHORYLATION ; ISOFORMS ; BINDING |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:A1997WX76200004 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274911]  |
| 专题 | 药理学第二研究室 |
| 作者单位 | CHINESE ACAD SCI, SHANGHAI INST MAT MED, DEPT PHARMACOL, SHANGHAI 200031, PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Zhai, R,Zhu, XZ. Production of anti-peptide antibody of rat brain nitric-oxide synthase[J]. ACTA PHARMACOLOGICA SINICA,1997,18(3):204-+. |
| APA | Zhai, R,&Zhu, XZ.(1997).Production of anti-peptide antibody of rat brain nitric-oxide synthase.ACTA PHARMACOLOGICA SINICA,18(3),204-+. |
| MLA | Zhai, R,et al."Production of anti-peptide antibody of rat brain nitric-oxide synthase".ACTA PHARMACOLOGICA SINICA 18.3(1997):204-+. |
入库方式： OAI收割
来源：上海药物研究所
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