Gsy, a novel glucansucrase from Leuconostoc mesenteroides, mediates the formation of cell aggregates in response to oxidative stress
文献类型：期刊论文
| 作者 | Yan, Minghui4,5; Han, Jin5; Xu, Xiaofen5; Liu, Lianliang1; Gao, Caixia5; Zheng, Huajun2; Chen, Yunxia3; Tao, Yimin6; Zhou, Hu6 ; Li, Yunfei4
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-12-07 |
| 卷号 | 6 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep38122 |
| 文献子类 | Article |
| 英文摘要 | Leuconostoc mesenteroides is a member of lactic acid bacteria (LAB) with wide applications in the food and medical industries. Species in the genus Leuconostoc are catalase-negative and generally regarded as facultative anaerobic or aerotolerant organisms. Despite their extensive use in industry, certain issues concerning the aerobic life of L. mesenteroides, e.g., the mechanism involved in the tolerance to oxygen, remain to be addressed. In this manuscript, a survival strategy employed by L. mesenteroides BD3749 in response to oxidative stress was elucidated. BD3749 cells cultivated in medium with sucrose available synthesized large amounts of exopolysaccharides, mostly consisting of insoluble EPS. When BD3749 cells were challenged with oxidative stress, the amount of insoluble EPS was greatly enhanced. The synthesized EPSs reduced the accumulation of reactive oxygen species (ROS) in bacterial cells and improved their survival during chronic oxidative stress. Another study showed that Gsy, a novel glucansucrase in the GH70 family that is induced by sucrose and up-regulated following exposure to oxygen, was responsible for the synthesis of insoluble EPS. Gsy was subsequently demonstrated to play pivotal roles in the formation of aggregates to alleviate the detrimental effects on BD3749 cells exerted by oxygen. |
| WOS关键词 | LACTIC-ACID BACTERIA ; 2 CATALYTIC DOMAINS ; SUPEROXIDE-DISMUTASE ; BIOFILM FORMATION ; MOLECULAR CHARACTERIZATION ; STREPTOCOCCUS-MUTANS ; LACTOCOCCUS-LACTIS ; NRRL B-1355 ; DSR-E ; DEXTRANSUCRASE |
| 资助项目 | National High Technology Research and Development Program of China (863 Program)[2011AA100901] ; National Science & Technology Pillar Program during the 12th Fiveyear Plan Period[2013BAD18B01] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000389690600001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275768]  |
| 专题 | 药物安全性评价中心 分析化学研究室 |
| 通讯作者 | Wu, Zhengjun |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol Minist Educ, Ningbo 315211, PR, Peoples R China 2.Chinese Natl Human Genome Ctr, Shanghai MOST Key Lab Hlth & Dis Genom, 250 Bi Bo Rd, Shanghai, Shanghai, Peoples R China; 3.Jiangnan Univ, Sch Food Sci & Technol, 1800 Lihu Rd, Wuxi 214122, PR, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Food Sci & Technol, Shanghai 200240, PR, Peoples R China; 5.Inst Bright Dairy Food Co Ltd, State Key Lab Dairy Biotechnol, 1518 West Jiangchang Rd, Shanghai 200436, PR, Peoples R China; 6.Shanghai Inst Mat Medica, Chinese Acad Sci, Dept Analyt Chem, Shanghai 201203, PR, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yan, Minghui,Han, Jin,Xu, Xiaofen,et al. Gsy, a novel glucansucrase from Leuconostoc mesenteroides, mediates the formation of cell aggregates in response to oxidative stress[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Yan, Minghui.,Han, Jin.,Xu, Xiaofen.,Liu, Lianliang.,Gao, Caixia.,...&Wu, Zhengjun.(2016).Gsy, a novel glucansucrase from Leuconostoc mesenteroides, mediates the formation of cell aggregates in response to oxidative stress.SCIENTIFIC REPORTS,6. |
| MLA | Yan, Minghui,et al."Gsy, a novel glucansucrase from Leuconostoc mesenteroides, mediates the formation of cell aggregates in response to oxidative stress".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：上海药物研究所
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