Chlorogenic acid analogues from Gynura nepalensis protect H9c2 cardiomyoblasts against H2O2-induced apoptosis
文献类型：期刊论文
| 作者 | Yu, Bang-wei2,3; Li, Jin-long1,3; Guo, Bin-bin1,3; Fan, Hui-min4; Zhao, Wei-min3 ; Wang, He-yao3
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| 刊名 | ACTA PHARMACOLOGICA SINICA
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| 出版日期 | 2016-11 |
| 卷号 | 37期号:11页码:1413-1422 |
| 关键词 | chlorogenic acid analogues Gynura nepalensis cardiomyocytes oxidative stress ROS apoptosis epigallocatechin gallate ERK/JNK pathway |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2016.79 |
| 文献子类 | Article |
| 英文摘要 | Aim: Chlorogenic acid has shown protective effect on cardiomyocytes against oxidative stress-induced damage. Herein, we evaluated nine caffeoylquinic acid analogues (1-9) isolated from the leaves of Gynura nepalensis for their protective effect against H2O2-induced H9c2 cardiomyoblast damage and explored the underlying mechanisms. Methods: H9c2 cardiomyoblasts were exposed to H2O2 (0.3 mmol/L) for 3 h, and cell viability was detected with MTT assay. Hoechst 33342 staining was performed to evaluate cell apoptosis. MMPs (mitochondrial membrane potentials) were measured using a JC-1 assay kit, and ROS (reactive oxygen species) generation was measured using CM-H(2)DCFDA. The expression levels of relevant proteins were detected using Western blot analysis. Results: Exposure to H2O2 markedly decreased the viability of H9c2 cells and catalase activity, and increased LDH release and intracellular ROS production; accompanied by a loss of MMP and increased apoptotic rate. Among the 9 chlorogenic acid analogues as well as the positive control drug epigallocatechin gallate (EGCG) tested, compound 6 (3,5-dicaffeoylquinic acid ethyl ester) was the most effective in protecting H9c2 cells from H2O2-induced cell death. Pretreatment with compound 6 (1.56-100 mu mol/L) dose-dependently alleviated all the H2O2-induced detrimental effects. Moreover, exposure to H2O2 significantly increased the levels of Bax, p53, cleaved caspase-8, and cleaved caspase-9, and decreased the level of Bcl-2, resulting in cell apoptosis. Exposure to H2O2 also significantly increased the phosphorylation of p38, JNK and ERK in the H9c2 cells. Pretreatment with compound 6 (12.5 and 25 mu mol/L) dose-dependently inhibited the H2O2-induced increase in the level of cleaved caspase-9 but not of cleaved caspase-8. It also dose-dependently suppressed the H2O2-induced phosphorylation of JNK and ERK but not that of p38. Conclusion: Compound 6 isolated from the leaves of Gynura nepalensis potently protects H9c2 cardiomyoblasts against H2O2-induced apoptosis, possibly by inhibiting intrinsic apoptosis and the ERK/JNK pathway. |
| WOS关键词 | HYDROGEN-PEROXIDE ; CELL-DEATH ; SIGNAL-TRANSDUCTION ; OXIDATIVE STRESS ; CARDIOMYOCYTES ; PATHWAY ; ACTIVATION ; HEART ; MITOCHONDRIA ; REPERFUSION |
| 资助项目 | National Natural Science Foundation of China[81473262] ; National Natural Science Foundation of China[81503124] ; Shanghai Three-year Plan on Promoting TCM Development[ZY3-LCPT-2-1003] ; Science and Technology Commission of Shanghai Municipality Project[15431901000] ; Science and Technology Commission of Shanghai Municipality Project[14ZR1447700] ; SIMM Projects[CASIMM0120162025] ; SIMM Projects[CASIMM0120164014] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5841408 |
| WOS记录号 | WOS:000387755900003 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275834]  |
| 专题 | 天然药物化学研究室 药理学第三研究室 |
| 通讯作者 | Fan, Hui-min; Zhao, Wei-min; Wang, He-yao |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Nanchang Univ, Coll Pharm, Nanchang 330038, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 4.Tongji Univ, Shanghai East Hosp, Sch Med, Shanghai 200120, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yu, Bang-wei,Li, Jin-long,Guo, Bin-bin,et al. Chlorogenic acid analogues from Gynura nepalensis protect H9c2 cardiomyoblasts against H2O2-induced apoptosis[J]. ACTA PHARMACOLOGICA SINICA,2016,37(11):1413-1422. |
| APA | Yu, Bang-wei,Li, Jin-long,Guo, Bin-bin,Fan, Hui-min,Zhao, Wei-min,&Wang, He-yao.(2016).Chlorogenic acid analogues from Gynura nepalensis protect H9c2 cardiomyoblasts against H2O2-induced apoptosis.ACTA PHARMACOLOGICA SINICA,37(11),1413-1422. |
| MLA | Yu, Bang-wei,et al."Chlorogenic acid analogues from Gynura nepalensis protect H9c2 cardiomyoblasts against H2O2-induced apoptosis".ACTA PHARMACOLOGICA SINICA 37.11(2016):1413-1422. |
入库方式： OAI收割
来源：上海药物研究所
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