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Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling

文献类型：期刊论文

作者Liu, Xing2,3; Xu, Yanli1; Meng, Qian2,3; Zheng, Qingqing4; Wu, Jianhong2,3; Wang, Chen5; Jia, Weiping5; Figeys, Daniel6,7; Chang, Ying4; Zhou, Hu2,3
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2016-08-05
卷号476期号:4页码:286-292
关键词Centrifugal proteomic reactor Label-free proteomics Minute amount Colorectal cancer Endoscopy
ISSN号0006-291X
DOI10.1016/j.bbrc.2016.05.114
文献子类Article
英文摘要Colorectal cancer (CRC) is one of the most common types of malignant tumor worldwide. Currently, although many researchers have been devoting themselves in CRC studies, the process of locating biomarkers for CRC early diagnosis and prognostic is still very slow. Using a centrifugal proteomic reactor based proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling, 2620 protein groups were quantified between cancer mucosa and adjacent normal colorectal mucosa. Of which, 403 protein groups were differentially expressed with statistic significance between cancer and normal tissues, including 195 up-regulated and 208 down-regulated proteins in cancer tissues. Three proteins (SOD3, PRELP and NGAL) were selected for further Western blot validation. And the resulting Western blot experimental results were consistent with the quantitative proteomic data. SOD3 and PRELP are down-regulated in CRC mucosa comparing to adjacent normal tissue, while NGAL is up-regulated in CRC mucosa. In conclusion, the centrifugal proteomic reactor-based label-free quantitative proteomic approach provides a highly sensitive and powerful tool for analyzing minute protein sample from tiny colorectal biopsies, which may facilitate CRC biomarkers discovery for diagnoses and prognoses. (C) 2016 Published by Elsevier Inc.
WOS关键词EXTRACELLULAR-SUPEROXIDE DISMUTASE ; MASS-SPECTROMETRY ; GENE-EXPRESSION ; CANCER ; CARCINOMA ; IDENTIFICATION ; CELLS ; INVASION
资助项目National Natural Science Foundation of China[21375138] ; Ministry of Science and Technology of China[2013ZX09507001] ; "one hundred talent program" of Chinese Academy of Sciences[00000000] ; Innovation Project of Instrument and Equipment Function Development[YZ201542] ; Bureau of Goods[00000000] ; Chinese Academy of Sciences[00000000] ; Natural Science for Youth Foundation of China[31500110] ; National Natural Science Foundation of China Outstanding Youth Fund[81322010]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
语种英语
WOS记录号WOS:000379886500017
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/275931]  
专题分析化学研究室
通讯作者Chang, Ying; Zhou, Hu
作者单位1.Fuyang Peoples Hosp, Fuyang, Peoples R China;
2.Chinese Acad Sci, Dept Analyt Chem, 555 Zuchongzhi Rd, Beijing 100864, Peoples R China;
3.Chinese Acad Sci, CAS Key Lab Receptor Res, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Beijing 100864, Peoples R China;
4.Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Digest Endoscop Ctr, 600 Yishan Rd, Shanghai 200233, Peoples R China;
5.Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Shanghai Clin Ctr Diabet,Shanghai Diabet Inst, Shanghai Key Lab Diabet Mellitus,Dept Endocrinol, Shanghai, Peoples R China;
6.Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1N 6N5, Canada;
7.Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON K1N 6N5, Canada
推荐引用方式
GB/T 7714		 Liu, Xing,Xu, Yanli,Meng, Qian,et al. Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,476(4):286-292.
APA Liu, Xing.,Xu, Yanli.,Meng, Qian.,Zheng, Qingqing.,Wu, Jianhong.,...&Zhou, Hu.(2016).Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,476(4),286-292.
MLA Liu, Xing,et al."Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 476.4(2016):286-292.

入库方式： OAI收割

来源：上海药物研究所

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