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Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic beta-cells in vitro

文献类型：期刊论文

作者Sun, Peng2,3; Wang, Ting3; Chen, Lu2; Yu, Bang-wei3; Jia, Qi2; Chen, Kai-xian2,3; Fan, Hui-min1; Li, Yi-ming2; Wang, He-yao3
刊名ACTA PHARMACOLOGICA SINICA
出版日期2016-08
卷号37期号:8页码:1083-1090
关键词cinnamon procyanidin oligomers cinnamtannin B1 procyanidin C1 cinnamtannin D1 diabetes pancreatic beta-cells cultured murine islets lipotoxicity ROS insulin secretion
ISSN号1671-4083
DOI10.1038/aps.2016.29
文献子类Article
英文摘要Aim: Cinnamon extracts rich in procyanidin oligomers have shown to improve pancreatic beta-cell function in diabetic db/db mice. The aim of this study was to identify the active compounds in extracts from two species of cinnamon responsible for the pancreatic beta-cell protection in vitro. Methods: Cinnamon extracts were prepared from Cinnamomum tamala (CT-E) and Cinnamomum cassia (CC-E). Six compounds procyanidin B2 (cpd1), (-)-epicatechin (cpd2), cinnamtannin B1 (cpd3), procyanidin C1 (cpd4), parameritannin A1 (cpd5) and cinnamtannin D1 (cpd6) were isolated from the extracts. INS-1 pancreatic beta-cells were exposed to palmitic acid (PA) or H2O2 to induce lipotoxicity and oxidative stress. Cell viability and apoptosis as well as ROS levels were assessed. Glucose-stimulated insulin secretion was examined in PA-treated beta-cells and murine islets. Results: CT-E, CC-E as well as the compounds, except cpd5, did not cause cytotoxicity in the beta-cells up to the maximum dosage using in this experiment. CT-E and CC-E (12.5-50 mu g/mL) dose-dependently increased cell viability in both PA- and H2O2-treated beta-cells, and decreased ROS accumulation in H2O2-treated beta-cells. CT-E caused more prominent beta-cell protection than CC-E. Furthermore, CT-E (25 and 50 mu g/mL) dose-dependently increased glucose-stimulated insulin secretion in PA-treated beta-cells and murine islets, but CC-E had little effect. Among the 6 compounds, trimer procyanidins cpd3, cpd4 and cpd6 (12.5-50 mu mol/L) dose-dependently increased the cell viability and decreased ROS accumulation in H2O2-treated beta-cells. The trimer procyanidins also increased glucose-stimulated insulin secretion in PA-treated beta-cells. Conclusion: Trimer procyanidins in the cinnamon extracts contribute to the pancreatic beta-cell protection, thus to the anti-diabetic activity.
WOS关键词INDUCED DIABETIC-RATS ; INSULIN-SECRETION ; DB/DB MICE ; GLUCOSE ; TYPE-2 ; VIVO ; POLYPHENOLS ; LIPIDS
资助项目National Natural Science Foundation[81473262] ; National Natural Science Foundation[21172044] ; National Science and Technology Major Project (Key New Drug Creation and Manufacturing Program)[2012ZX09301001-001] ; National Science and Technology Major Project (Key New Drug Creation and Manufacturing Program)[2013ZX09103001-001] ; Natural Science Foundation of Shanghai, China[15ZR1441200] ; Shanghai Three-year Plan on Promoting TCM Development[ZY3-LCPT-2-1003]
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
CSCD记录号CSCD:5766618
WOS记录号WOS:000380927200008
出版者ACTA PHARMACOLOGICA SINICA
源URL[http://119.78.100.183/handle/2S10ELR8/275939]  
专题天然药物化学研究室
药理学第三研究室
上海中药现代化研究中心
通讯作者Li, Yi-ming; Wang, He-yao
作者单位1.Tongji Univ, Dept Cardiovasc & Thorac Surg, Shanghai East Hosp, Shanghai Heart Failure Res Ctr,Sch Med, Shanghai 200123, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Sun, Peng,Wang, Ting,Chen, Lu,et al. Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic beta-cells in vitro[J]. ACTA PHARMACOLOGICA SINICA,2016,37(8):1083-1090.
APA Sun, Peng.,Wang, Ting.,Chen, Lu.,Yu, Bang-wei.,Jia, Qi.,...&Wang, He-yao.(2016).Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic beta-cells in vitro.ACTA PHARMACOLOGICA SINICA,37(8),1083-1090.
MLA Sun, Peng,et al."Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic beta-cells in vitro".ACTA PHARMACOLOGICA SINICA 37.8(2016):1083-1090.

入库方式： OAI收割

来源：上海药物研究所

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