Quantitative proteomic analysis of mice corneal tissues reveals angiogenesis-related proteins involved in corneal neovascularization
文献类型：期刊论文
| 作者 | Shen, Minqian3; Tao, Yimin1,2,4,5; Feng, Yifan3; Liu, Xing1,2,4,5; Yuan, Fei3; Zhou, Hu1,2,4,5
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| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
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| 出版日期 | 2016-07 |
| 卷号 | 1864期号:7页码:787-793 |
| 关键词 | Alkali burn Corneal neovascularization Mice Proteomics |
| ISSN号 | 1570-9639 |
| DOI | 10.1016/j.bbapap.2016.04.001 |
| 文献子类 | Article |
| 英文摘要 | Corneal neovascularization (CNV) was induced in Balb/c mice by alkali burns in the central area of the cornea with a diameter of 2.5 mm. After fourteen days, the cornea from one eye was collected for histological staining for CNV examination, while the cornea from the other eye of the same mouse was harvested for proteomic analysis. The label-free quantitative proteomic approach was applied to analyze five normal corneal tissues (normal group mice n = 5) and five corresponding neovascularized corneal tissues (model group mice n = 5). A total of 2124 proteins were identified, and 1682 proteins were quantified from these corneal tissues. Among these quantified proteins, 290 proteins were significantly changed between normal and alkali burned corneal tissues. Of these significantly changed proteins, 35 were reported or predicted as angiogenesis-related proteins. Then, these 35 proteins were analyzed using Ingenuity Pathway Analysis Software, resulting in 26 proteins enriched and connected,to each other in the protein-protein interaction network, such as Lcn-2, alpha B-crystallin and Serpinf1 (PEDF). These three significantly changed proteins were selected for further Western blotting validation. Consistent with the quantitative proteomic results, Western blotting showed that Lcn-2 and aB-crystallin were significantly up-regulated in CNV model, while PEDF was down-regulated. This study provided increased understanding of angiogenesis-related proteins involved in corneal vascular development, which will be useful in the ophthalmic clinic of specifically target angiogenesis. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | ALPHA-B-CRYSTALLIN ; EPITHELIUM-DERIVED FACTOR ; ENDOTHELIAL GROWTH-FACTOR ; PROLIFERATIVE DIABETIC-RETINOPATHY ; BREAST-CANCER ; HEPATOCELLULAR-CARCINOMA ; MESENCHYMAL TRANSITION ; LIPOCALIN 2 ; EXPRESSION ; CELLS |
| 资助项目 | National Natural Science Foundation of China[21375138] ; National Natural Science Foundation of China[81470637] ; National Natural Science Foundation of China[31300681] ; "one hundred talent program" of Chinese Academy of Sciences[00000000] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2013ZX09507001] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000376706900005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275981]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Yuan, Fei; Zhou, Hu |
| 作者单位 | 1.Univ Ottawa, Ottawa, ON K1N 6N5, Canada 2.Chinese Acad Sci, SIMMUOMICS Lab, Joint Res Lab Translat OMICS, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Fudan Univ, Zhongshan Hosp, Dept Ophthalmol, 180 Fenglin Rd, Shanghai 200032, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Shen, Minqian,Tao, Yimin,Feng, Yifan,et al. Quantitative proteomic analysis of mice corneal tissues reveals angiogenesis-related proteins involved in corneal neovascularization[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2016,1864(7):787-793. |
| APA | Shen, Minqian,Tao, Yimin,Feng, Yifan,Liu, Xing,Yuan, Fei,&Zhou, Hu.(2016).Quantitative proteomic analysis of mice corneal tissues reveals angiogenesis-related proteins involved in corneal neovascularization.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1864(7),787-793. |
| MLA | Shen, Minqian,et al."Quantitative proteomic analysis of mice corneal tissues reveals angiogenesis-related proteins involved in corneal neovascularization".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1864.7(2016):787-793. |
入库方式： OAI收割
来源：上海药物研究所
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