Cytotoxic and Pro-Apoptotic Effects of Cassane Diterpenoids from the Seeds of Caesalpinia sappan in Cancer Cells
文献类型：期刊论文
| 作者 | Bao, Han1; Zhang, Le-Le1; Liu, Qian-Yu1; Feng, Lu2; Ye, Yang2 ; Lu, Jin-Jian1; Lin, Li-Gen1
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| 刊名 | MOLECULES
 |
| 出版日期 | 2016-06 |
| 卷号 | 21期号:6 |
| 关键词 | Caesalpinia sappan cassane diterpenoids cytotoxicity apoptosis p53 |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules21060791 |
| 文献子类 | Article |
| 英文摘要 | The chemical study on the seeds of Caesalpinia sappan led to the isolation of five new cassane diterpenoids, phanginins R-T (1-3) and caesalsappanins M and N (4 and 5), together with seven known compounds 6-12. Their structures were elucidated on the basis of NMR and HRESIMS analyses. The absolute configurations of compounds 1 and 4 were determined by the corresponding CD spectra. All the isolated compounds were tested for their cytotoxicity against ovarian cancer A2780 and HEY, gastric cancer AGS, and non-small cell lung cancer A549 cells. Compound 1 displayed significant toxicity against the four cell lines with the IC50 values of 9.9 +/- 1.6 mu M, 12.2 +/- 6.5 mu M, 5.3 +/- 1.9 mu M, and 12.3 +/- 3.1 mu M, respectively. Compound 1 induced G1 phase cell cycle arrest in A2780 cells. Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. What's more, compound 1 also promoted the expression of the tumor suppressor p53 protein. These findings indicate that cassane diterpenoids might have potential as anti-cancer agents, and further in vivo animal studies and structural modification investigation are needed. |
| WOS关键词 | ECHINATA LEGUMINOSAE ; MINAX ; FURANODITERPENES ; CONSTITUENTS ; HEARTWOOD ; ROOTS ; LINN ; P53 |
| 资助项目 | Science and Technology Development Fund[00000000] ; Macao S.A.R[FDCT 042/2013/A2] ; University of Macau[MYRG2014-00020-ICMS-QRCM] ; University of Macau[MYRG2015-00153-ICMS-QRCM] ; University of Macau[MYRG2015-00091-ICMS-QRCM] ; University of Macau[MYRG2015-00101-ICMS-QRCM] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000378757600117 |
| 出版者 | MDPI AG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276015]  |
| 专题 | 天然药物化学研究室 |
| 通讯作者 | Lu, Jin-Jian; Lin, Li-Gen |
| 作者单位 | 1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Nat Prod Chem, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Han,Zhang, Le-Le,Liu, Qian-Yu,et al. Cytotoxic and Pro-Apoptotic Effects of Cassane Diterpenoids from the Seeds of Caesalpinia sappan in Cancer Cells[J]. MOLECULES,2016,21(6). |
| APA | Bao, Han.,Zhang, Le-Le.,Liu, Qian-Yu.,Feng, Lu.,Ye, Yang.,...&Lin, Li-Gen.(2016).Cytotoxic and Pro-Apoptotic Effects of Cassane Diterpenoids from the Seeds of Caesalpinia sappan in Cancer Cells.MOLECULES,21(6). |
| MLA | Bao, Han,et al."Cytotoxic and Pro-Apoptotic Effects of Cassane Diterpenoids from the Seeds of Caesalpinia sappan in Cancer Cells".MOLECULES 21.6(2016). |
入库方式： OAI收割
来源：上海药物研究所
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