Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome
文献类型：期刊论文
| 作者 | Shi, Yuan3; Chen, Xiang4; Elsasser, Suzanne3; Stocks, Bradley B.5; Tian, Geng3; Lee, Byung-Hoon3; Shi, Yanhong4,6 ; Zhang, Naixia6; de Poot, Stefanie A. H.3; Tuebing, Fabian3
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| 刊名 | SCIENCE
 |
| 出版日期 | 2016-02-19 |
| 卷号 | 351期号:6275 |
| ISSN号 | 0036-8075 |
| DOI | 10.1126/science.aad9421 |
| 文献子类 | Article |
| 英文摘要 | Hundreds of pathways for degradation converge at ubiquitin recognition by a proteasome. Here, we found that the five known proteasomal ubiquitin receptors in yeast are collectively nonessential for ubiquitin recognition and identified a sixth receptor, Rpn1. A site (T1) in the Rpn1 toroid recognized ubiquitin and ubiquitin-like (UBL) domains of substrate shuttling factors. T1 structures with monoubiquitin or lysine 48 diubiquitin show three neighboring outer helices engaging two ubiquitins. T1 contributes a distinct substrate-binding pathway with preference for lysine 48-linked chains. Proximal to T1 within the Rpn1 toroid is a second UBL-binding site (T2) that assists in ubiquitin chain disassembly, by binding the UBL of deubiquitinating enzyme Ubp6. Thus, a two-site recognition domain intrinsic to the proteasome uses distinct ubiquitin-fold ligands to assemble substrates, shuttling factors, and a deubiquitinating enzyme. |
| WOS关键词 | MULTIUBIQUITIN-CHAIN-BINDING ; EXCHANGE MASS-SPECTROMETRY ; 26S PROTEASOME ; SACCHAROMYCES-CEREVISIAE ; UBIQUITIN RECEPTORS ; PROTEIN DDI1 ; H/D EXCHANGE ; CYCLIN B1 ; DEGRADATION ; SUBUNIT |
| 资助项目 | National Institutes of Health[R37-GM043601] ; National Institutes of Health[CA136472] ; National Institutes of Health[R01-GM101135] ; NIH, National Cancer Institute, Center for Cancer Research[00000000] ; Waters Corporation[00000000] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
| WOS记录号 | WOS:000370325700037 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276141]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Engen, John R.; Finley, Daniel; Walters, Kylie J. |
| 作者单位 | 1.Linganore High Sch, Frederick, MD 21701 USA; 2.NCI, Biophys Resource, Struct Biophys Lab, Ctr Canc Res, Frederick, MD 21702 USA 3.Harvard Univ, Sch Med, Dept Cell Biol, 240 Longwood Ave, Boston, MA 02115 USA; 4.NCI, Prot Proc Sect, Struct Biophys Lab, Ctr Canc Res, Frederick, MD 21702 USA; 5.Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA; 6.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Shi, Yuan,Chen, Xiang,Elsasser, Suzanne,et al. Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome[J]. SCIENCE,2016,351(6275). |
| APA | Shi, Yuan.,Chen, Xiang.,Elsasser, Suzanne.,Stocks, Bradley B..,Tian, Geng.,...&Walters, Kylie J..(2016).Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome.SCIENCE,351(6275). |
| MLA | Shi, Yuan,et al."Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome".SCIENCE 351.6275(2016). |
入库方式： OAI收割
来源：上海药物研究所
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